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Product Description
Affinity-purified polyclonal sheep IgG directed against host cell proteins (HCPs) from MDCK (Madin-Darby Canine Kidney) cells. Immunogen: MDCK HCPs produced by sonication cell lysis. Provided as an unconjugated liquid antibody in PBS without preservatives. Supplied at 1 mg total protein. Intended for research use applications including HCP coverage evaluations, Western blot and ELISA.
Technical Specifications
| Parameter | Details |
|---|---|
| Size | 1 mg |
| Species Reactivity | Canis familiaris (dog) |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody (unconjugated) |
| Conjugate | Unconjugated |
| Immunogen | MDCK HCPs produced by sonication cell lysis |
| Form | Liquid |
| Purification | Affinity Purified |
| Buffer | PBS |
| Contains | No preservatives |
| Applications | HCP coverage evaluations, Western Blot, ELISA |
| Storage | Maintain at 2-8 °C for short-term use. For long-term storage store below -65 °C. |
| Validity period | 12 months |
Features
- Broad MDCK HCP Recognition: Polyclonal antibody raised against whole MDCK host cell protein lysate for broad coverage of HCP species.
- Affinity Purified: Affinity purification improves specificity and reduces background in immunoassays.
- Unconjugated Format: Flexible for use with a variety of secondary detection systems (HRP, AP, fluorescent labels) and assay formats.
- Ready-to-use Liquid: Supplied in PBS as a liquid preparation for immediate use in assays; no reconstitution required.
- No Preservatives: Formulation without preservatives suitable for many downstream analytical workflows (store appropriately to prevent contamination).
Applications
- HCP coverage evaluations
- Western Blot
- ELISA
- General research immunoassays against MDCK host cell proteins
Kit Contents
Anti-MDCK HCP Antibody (Affinity-purified, unconjugated)Maintain at 2-8 °C for short-term; for long-term store below -65 °C
* Total: Not applicable (antibody reagent) | Method: Immunoassay (ELISA / Western Blot)
Attention
• For research use only (RUO). Not for diagnostic or therapeutic use.
• Maintain at 2-8 °C for short-term storage. For long-term storage, keep below -65 °C.
• Avoid repeated freeze–thaw cycles; aliquot if necessary.
• Contains no preservatives—use aseptic technique to avoid contamination.
• Follow institutional safety procedures for handling biological reagents and animal-derived materials.
Quality Management & Certifications
Quality System
Download QMSQMS (ISO, GMP)
Download CertificateQuality Advantages
View ReportQuality Control Process
Download ProcessTechnical Resources & Downloads
Frequently Asked Questions (FAQ)
Q1: What species was this antibody raised in and what is the isotype?
This antibody is a polyclonal raised in sheep and is of the IgG isotype.
Q2: What applications is this antibody validated for?
The product is intended for HCP coverage evaluations and is suitable for Western blot and ELISA. Users should optimize conditions for their specific assay.
Q3: How should the antibody be stored and what is its shelf life?
Store at 2-8 °C for short-term use. For long-term storage keep below -65 °C. The validated shelf life is 12 months.
Q4: Is this product suitable for diagnostic use?
No. This product is for research use only (RUO) and is not intended for diagnostic purposes.
Research Use Only
✔
Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.
✔
Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
