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Product Description
SHENTEK® Virus DNA & RNA Extraction Kit is a magnetic-particle based kit designed for isolation of viral DNA and RNA from cell samples (up to 10^7 cells) and harvested virus bulk (liquid). The kit supports both manual workflows and automated extraction with the SHENTEK® rHCDpurify system. Two sample workflows are provided: Method 1 (for viruses replicated in host nucleus or unknown viruses) includes an optional nuclease digestion step after lysis to reduce background nucleic acids; Method 2 (for viruses replicated in host cytoplasm) uses lysis followed by centrifugation and extraction of the supernatant. The kit contains protease digestion, binding, wash and elution reagents and magnetic particles to deliver purified nucleic acids suitable for downstream molecular assays (e.g., PCR/qPCR, sequencing). The kit is supplied for 50 extractions.
Technical Specifications
| Parameter | Details |
|---|---|
| Pack size | Reagents for 50 extractions |
| Product number | 1506730 |
| Sample input capacity | Up to 1 × 10^7 cells; maximum sample volume per well up to 500 µL (automated plate format) |
| Eluate volume | 50–100 µL RNase-free H2O (preheated at 70 °C); ensure >40 µL for downstream assay |
| Binding reagents per sample | 200 µL binding buffer + 200–400 µL isopropanol + 30 µL magnetic particles |
| Wash volumes | Wash buffer A: 700 µL; Wash buffer B: 700 µL |
| Key incubation temperatures & times | Protease digestion: 55 °C for 30 min; Nuclease digestion (optional): 37 °C for 30 min; Elution: 70 °C for 7 min; Magnetic particles incubation at RT 10 min |
| Magnetic particle handling | Incubate magnetic particles at RT 10 min and vortex to mix before use; aliquot to avoid repeated temperature shifts |
| Maximum sample volume (plate) | Maximum per deep-well plate well: 500 µL |
| Storage stability | Kit components stable for up to 24 months (see individual component storage below) |
| Compatibility | Manual extraction or automated extraction using SHENTEK® rHCDpurify system; downstream compatible with PCR/qPCR and sequencing workflows |
Features
- Dual DNA & RNA extraction: Simultaneous isolation of both viral DNA and RNA from the same sample using magnetic-particle chemistry.
- Two-method workflow: Method 1 includes nuclease digestion for nucleus-replicating or unknown viruses to reduce background; Method 2 optimized for cytoplasm-replicating viruses (centrifugation of lysate).
- Manual and automated operation: Validated for manual tube-based workflows and automated high-throughput extraction with the SHENTEK® rHCDpurify system (96-deep well plate layout supported).
- Magnetic particle technology: Magnetic beads provide reliable binding, washing and elution for high-quality nucleic acids suitable for downstream molecular assays.
- Ready for downstream molecular assays: Eluted nucleic acids are suitable for PCR/qPCR and sequencing; recommended to perform detection assays the same day for best accuracy.
- Long shelf life: Kit reagents stable for up to 24 months when stored under recommended conditions.
Applications
- Extraction of viral DNA and RNA from cell culture samples (up to 1×10^7 cells)
- Extraction of nucleic acids from harvested virus bulk (liquid)
- Sample preparation for PCR and qPCR assays
- Preparation of viral nucleic acids for sequencing or downstream molecular testing
- Processing of rcAAV/rAAV, replication competent lentivirus (RCL), replication competent retrovirus (RCR) and RNA viruses
Kit Contents
Wash buffer A (NND015)Room temperature (15 mL × 1 bottle)
Binding solution (NND017)Room temperature (10 mL × 1 bottle)
Proteinase K Buffer (NND025)Room temperature (5 mL × 1 bottle)
Pretreatment buffer (NND002)Room temperature (1.25 mL × 2 tubes)
Magnetic particles (NND030)2–8 °C (750 µL × 2 tubes)
5M NaCl (NND040)Room temperature (500 µL × 1 tube)
Precipitation solution I (NND003)Room temperature (25 µL × 1 tube) — to be diluted 1:99 with RNase-Free H2O before use
RNase-Free H2O (NND008)-20 °C (1.2 mL × 6 bottles)
Proteinase K (NND023)Store per label (500 µL × 5 tubes) — follow kit label/insert
* Total: 50 extractions | Method: Magnetic bead-based nucleic acid extraction (manual or automated with rHCDpurify)
Attention
• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (eye protection, masks, gloves, lab coat).
• Separate laboratory areas for reagent preparation, sample preparation and amplification to avoid contamination.
• Ensure ambient temperature ≥ 22 °C at experiment start.
• Change gloves regularly and use dedicated protective equipment for different lab areas.
• Centrifuge tubes before opening to avoid aerosols; spin down liquid on tube caps/walls.
• Discard and disinfect liquid waste and used tips according to lab biosafety procedures.
• Do not over-dry magnetic particle pellet; over-drying may hinder resuspension during elution.
• Place tubes on magnetic stand with pellet oriented toward the magnet and rotate tubes slowly to accelerate bead aggregation.
• After extraction, perform DNA/RNA detection assays preferably the same day for accurate results.
• For automated runs with rHCDpurify: perform UV sterilization for at least 15 minutes and wipe interior with 75% ethanol before and after runs; maintain at least 30 min between extractions.
Quality Management & Certifications
Quality System
Download QMSQMS (ISO, GMP)
Download CertificateQuality Advantages
View ReportQuality Control Process
Download ProcessTechnical Resources & Downloads
Frequently Asked Questions (FAQ)
Q1: Can this kit isolate both viral DNA and RNA?
Yes. The SHENTEK® Virus DNA & RNA Extraction Kit is formulated to extract both viral DNA and RNA from cell samples and harvested virus liquid.
Q2: How many samples can be processed with one kit?
The supplied kit contains reagents for 50 extractions.
Q3: Is the kit compatible with automation?
Yes. The kit is compatible with manual workflows and automated extraction using the SHENTEK® rHCDpurify system (96-deep well plate format).
Q4: What elution volume and conditions are recommended?
Elute with 50–100 µL RNase-Free H2O preheated to 70 °C and incubate at 70 °C for 7 minutes; ensure at least ~40 µL final elute for downstream assays.
Research Use Only
✔
For Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.
✔
Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
