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Product Description
The SHENTEK® MVM Detection Kit is a qualitative real-time PCR assay for detection of Minute Virus of Mice (MVM) contamination, covering MVM strains i, p, m and c, in mouse-derived cell lines and related sample matrices used in biopharmaceutical production (examples: CHO, BHK, NS0). The kit is designed for use with the SHENTEK® Virus DNA & RNA Extraction Kit (Product No. 1506730). The assay includes MVM-specific primer/probe mix, internal process control (VIR Internal Control), and positive control to monitor extraction and amplification. Combined with SHENTEK extraction, the kit enables efficient recovery and sensitive detection of MVM DNA from complex matrices such as cells and serum. The assay demonstrates high specificity (no cross-amplification observed with tested cell lines and microbial genomes) and a reported analytical sensitivity of 50 copies per reaction. The kit is supplied for 50 reactions.
Technical Specifications
| Parameter | Details |
|---|---|
| Intended use | Qualitative detection of Minute Virus of Mice (MVM) strains i, p, m and c in mouse-derived cell lines and serum |
| Kit size | Reagents for 50 reactions |
| Analytical sensitivity (LOD) | 50 copies per reaction |
| Specificity | No cross-amplification observed with tested engineered cell lines (CHO, VERO, 293T, MDCK, NS0, Sf9) or engineered bacteria genomes (E. coli, Pichia pastoris) |
| Reaction volume | 30 µL total reaction volume (20 µL qPCR MIX + 10 µL purified sample / control / NTC) |
| qPCR mix composition (per reaction) | qPCR Master MIX 15.9 µL; MVM Primer & Probe MIX 2.4 µL; VIR IPC MIX 1.7 µL; (add 0.5 µL VIR IC to mix if not added during extraction); total qPCR MIX = 20 µL |
| Thermal cycling conditions | Activation: 95 °C 10:00; 40 cycles of: Denaturation 95 °C 00:15, Annealing/Extension 60 °C 01:00 (fluorescence read during annealing/extension) |
| Controls provided / required | VIR Internal Control (IC) included; Positive Control (MVM Positive Control) is referenced (Product No. 1506747) and recommended; Negative control (DDB or 1×PBS) and NTC required |
| Acceptance criteria — control wells | NTC: FAM undetected; VIC: Ct < 35 for duplicate runs. NCS: FAM undetected; VIC: Ct < 35. PCS: FAM Ct < 35 and VIC Ct < 35 |
| Acceptance criteria — test samples | Positive: FAM Ct < 40 (at least one well) with specific amplification and VIC Ct < 35 in duplicates. Negative: FAM undetected in duplicates and Ctsample - CtNCS < 2. Not conclusive / possible inhibition: Ctsample - CtNCS ≥ 2 (compare VIC Ct of sample to NCS); retest or remove inhibitors |
| Compatible instruments (examples) | SHENTEK-96S, LightCycler 480 II, ABI 7500 and most real-time PCR systems supporting probe assays |
| Storage & stability | Kit components stored under recommended conditions (primarily -20 °C where indicated). Components stable up to 24 months (check label expiration date) |
Features
- Broad MVM strain coverage: Targets and detects four MVM strains (i, p, m and c).
- High analytical sensitivity: Reported limit of detection of 50 copies per reaction.
- High specificity: No cross-amplification with common mammalian cell lines or tested microbial genomes.
- Internal process control included: VIR Internal Control (IC) monitors extraction and PCR inhibition.
- Supplied controls: MVM Positive Control provided (reference product number supplied separately); NTC and NCS workflows specified.
- Compatible with automated extraction: Recommended for use with rHCDpurify System and the SHENTEK® Virus DNA & RNA Extraction Kit to efficiently recover viral DNA from complex matrices.
- Ready-to-use primer/probe mix: Pre-mixed MVM primer & probe simplifies setup and reduces assay variability.
- Validated qPCR program: Predefined cycling conditions and instrument setup guidance provided for common platforms.
Applications
- Contamination screening of mouse-derived cell lines (e.g., CHO, BHK, NS0) used in biopharmaceutical production
- Testing of cell culture supernatant and serum for MVM DNA
- In-process and release testing support in research environments
- Research studies requiring qualitative detection of MVM
Kit Contents
VIR Internal Control (IC) (Part No. NNA057) — 600 µL × 1 tube-20 °C
DNA Dilution Buffer (DDB) (Part No. NND001) — 1.5 mL × 1 tubeRefer to label
qPCR Master MIX (Part No. NNB023) — 850 µL × 1 tube-20 °C, protect from light
MVM Primer & Probe MIX (Part No. NNC106) — 150 µL × 1 tube-20 °C
VIR IPC MIX (Part No. NNC107) — 100 µL × 1 tube-20 °C
* Total: 50 Reactions | Method: qPCR
Attention
• For Research Use Only — consult compliance statement.
• Read Material Safety Data Sheets (MSDS) and follow handling instructions.
• Wear appropriate PPE (protective eyewear, mask, clothing and gloves).
• Handle positive control and extraction in a biological safety cabinet and follow BSL-2 laboratory safety guidelines.
• Use nuclease-free consumables and certified reagents.
• Thaw kit components at 2–8 °C or on ice; vortex and briefly centrifuge before use.
• Avoid cross-contamination: use separate areas and aerosol-resistant tips for reagent preparation, sample handling, and amplification.
• If VIC internal control Ct of sample is >2 cycles different from NCS VIC Ct, suspect PCR inhibition; retest or treat sample to remove inhibitors.
• Check expiration dates on component labels and store components under appropriate conditions (see labels).
Quality Management & Certifications
Quality System
Download QMSQMS (ISO, GMP)
Download CertificateQuality Advantages
View ReportQuality Control Process
Download ProcessTechnical Resources & Downloads
Frequently Asked Questions (FAQ)
Q1: What is the limit of detection (LOD) of the SHENTEK MVM Detection Kit?
The reported analytical sensitivity is 50 copies per reaction.
Q2: Which MVM strains does this kit detect?
The assay is designed to detect MVM strains i, p, m and c.
Q3: What sample types are supported and what extraction is recommended?
The kit is intended for cell culture and serum samples (example: 200 µL sample input). Use the SHENTEK® Virus DNA & RNA Extraction Kit (Product No. 1506730) and rHCDpurify System for nucleic acid extraction as recommended.
Q4: How are results interpreted and what indicates inhibition?
Positive: FAM Ct < 40 (at least one well) with specific amplification and VIC Ct < 35. Negative: FAM undetected in duplicates and Ctsample - CtNCS < 2. If Ctsample - CtNCS ≥ 2 (VIC comparison), this suggests PCR inhibition; retest or remove inhibitors.
Research Use Only
✔
Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.
✔
Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
