0 item(s)
View cart
You have no items in your shopping cart.
For Research Use Only (RUO)
$3,070.00
Sku: 1504633
Product Description
MicroSHENTEK® Fungi & Bacteria DNA Extraction Kit is a magnetic-particle based nucleic acid extraction kit designed to isolate trace fungal and bacterial DNA from complex matrices (≤ 10^6 cells) including master cell banks, working cell banks, vaccines and cellular therapeutic products. The kit is intended to be used together with MicroSHENTEK® Fungi DNA Detection Kit and MicroSHENTEK® Bacteria DNA Detection Kit to provide an integrated workflow from sample preparation to qPCR detection. The kit is compatible with automated extraction on the rHCDpurify system and supports inclusion of an internal control for fungal extraction. Recommended system extraction performance is 50–100 CFU per tube, and the corresponding detection kit limit of detection is ≤35 CFU/reaction.
Technical Specifications
| Parameter | Details |
|---|---|
| Format / Capacity | Reagents for 50 extractions; Product No.: 1504633 |
| Recommended extraction performance | 50–100 CFU per tube |
| Corresponding detection kit LOD | ≤35 CFU per reaction (when used with MicroSHENTEK detection kits) |
| Sample input matrix | Master cell banks, working cell banks, vaccines, cellular therapeutic products and other complex matrices (≤10^6 cells) |
| Digestion conditions | Add 10 μL 5M NaCl, 10 μL Proteinase K and 100 μL Lysis buffer; incubate at 55 °C for 40–60 min (vortex and extend as needed up to complexity of matrix) |
| Cell wall fragmentation (bead mill) recommended settings | 5 m/s for 40 s, 1 cycle |
| Centrifugation | 16200×g, 4 °C for 15–30 min (sample enrichment) and ≥16200×g for at least 2 min after fragmentation |
| Automated extraction runtime | rHCDpurify Micro-633 program, approximately 52 minutes per run |
| Minimum interval between extractions | 30 minutes (to allow UV/cleaning between runs) |
| Storage stability | Up to 24 months (check labels for expiration) |
| Eluate volume requirement | Ensure >40 μL bottom eluate for downstream assay |
Features
- High sensitivity: Recommended extraction performance of 50–100 CFU/tube and compatible with detection kit LOD ≤35 CFU/reaction.
- Automation compatible: Validated for automated extraction using the rHCDpurify system (Micro-633 program, ~52 min).
- Broad sample compatibility: Optimized for complex matrices including master/working cell banks, vaccines and cell therapy products (≤10^6 cells).
- Integrated workflow: Designed to work together with MicroSHENTEK® Fungi and Bacteria DNA Detection Kits for direct downstream qPCR.
- Internal control support: Provides an option to add fungal internal control (IC) during sample preparation for extraction quality monitoring.
Applications
- Trace fungi and bacteria DNA extraction from master cell banks and working cell banks
- Sample preparation for microbial testing of vaccines and cellular therapeutic products
- Extraction from other complex biological matrices prior to qPCR-based microbial detection
- Automated high-throughput extraction on rHCDpurify system (96-deep well plate workflows)
Kit Contents
Lysis buffer (NND028)Room temperature; 5 mL × 2 bottles
Binding solution (NND017)Room temperature; 10 mL × 2 bottles
Wash buffer A (NND015)Room temperature; 15 mL × 2 bottles (add 20 mL anhydrous ethanol before use)
Elution buffer (NND019)Room temperature; 5 mL × 2 bottles
Dilution buffer (NND021)Room temperature; 10 mL × 2 bottles
Sample treatment tube (NND005)Room temperature; 50 tubes
5M NaCl (NND040)2–8 °C; 500 μL × 2 tubes
Magnetic particles (NND033)2–8 °C; 750 μL × 2 tubes (equilibrate to room temp and vortex before use)
Precipitation solution I (NND003)-20 °C; 25 μL × 2 tubes
Precipitation solution II (NND004)-20 °C; 500 μL × 2 tubes
Proteinase K (NND023)-20 °C; 500 μL × 2 tubes
* Total: 50 Extractions | Method: Automated magnetic particle-based DNA extraction (compatible with rHCDpurify); intended for downstream qPCR
Attention
• For research use only; read Material Safety Data Sheets (MSDS) and use appropriate PPE (eye protection, mask, gloves, lab coat).
• Separate laboratory areas for negative control/reagent prep, sample processing and positive control to avoid cross-contamination.
• Disinfect work areas with nucleic acid scavenger and 75% ethanol; perform UV irradiation of benches (≥1 hour) and work areas (≥30 minutes) before use.
• Before and after rHCDpurify runs perform UV sterilization for at least 15 minutes and wipe with 75% ethanol; minimum interval between extractions is 30 minutes.
• Maintain ambient experimental temperature ≥22 °C and humidity ≤70% during procedures.
• Change gloves and protective garments regularly and use different supplies in different areas to avoid contamination.
• Ensure magnetic particles are equilibrated and vortexed prior to use; if Lysis or Binding solutions appear cloudy, warm to 37 °C and mix until clear.
• After extraction immediately transfer purified eluate to a new tube and ensure at least 40 μL is recovered for downstream qPCR; perform qPCR the same day if possible.
Quality Management & Certifications
Quality System
Download QMSQMS (ISO, GMP)
Download CertificateQuality Advantages
View ReportQuality Control Process
Download ProcessTechnical Resources & Downloads
Frequently Asked Questions (FAQ)
Q1: What is the sensitivity of this extraction kit?
The recommended extraction performance is 50–100 CFU per tube; when used with the corresponding MicroSHENTEK detection kits the detection limit is ≤35 CFU per reaction.
Q2: How many extractions are provided per kit?
This kit contains reagents for 50 extractions.
Q3: What are the recommended storage conditions?
Kit components are stored at room temperature, 2–8 °C, or -20 °C depending on the component; overall stability up to 24 months. Check individual component labels for specific storage instructions.
Q4: Can this kit be used with an automated extractor?
Yes. The kit is compatible with the rHCDpurify system and the Micro-633 program (approx. 52 minutes run). Follow plate layouts and program settings in the user guide.
Research Use Only
✔
For Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.
✔
Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
