For Research Use Only (RUO)

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⚡ Detection Method
ELISA
🕒 Assay Time
Refer to manual
🎯 Storage Temperature
2-8 °C (short-term); < -65 °C (stable for 12 months)

Product Description

SHENTEK™ Anti-E.coli (K-12 & Alkaline Lysis) HCP Antibody is an affinity-purified polyclonal sheep IgG raised against Escherichia coli host cell proteins (HCPs) derived from K-12 strains prepared by alkaline lysis. The reagent is supplied as a liquid, unconjugated antibody in PBS without preservatives and is intended for research applications including HCP coverage evaluations, Western blot and ELISA. Supplied at 1 mg per vial. Store at 2-8 °C for short-term use and below -65 °C for long-term storage. This product is for research use only (RUO) and is not for diagnostic purposes.

Technical Specifications

Parameter Details
Product No. 1301302-Ab01
Size 1 mg
Species Reactivity Escherichia coli (E. coli), K-12 strains
Host / Isotype Sheep / IgG
Class Polyclonal
Type Antibody, unconjugated
Immunogen E. coli HCPs (K-12 strains) produced chemically by alkaline lysis
Form Liquid
Purification Affinity Purified
Storage buffer PBS
Contains No preservatives
Applications HCP Coverage evaluations, Western Blot, ELISA
Storage Maintain at 2-8 °C for short-term use. For long-term storage, store below -65 °C.
Validity period 12 months (when stored as recommended)

Features

  • Affinity-purified polyclonal antibody: Provides broad coverage of E. coli host cell proteins derived from K-12 strains.
  • Produced against alkaline-lysis HCPs: Immunogen produced by chemical alkaline lysis to represent HCPs from K-12 strains.
  • Unconjugated format: Suitable for use as primary antibody in ELISA, Western blot and HCP coverage evaluation workflows.
  • No preservatives: Formulated in PBS without preservatives to minimize interference in downstream assays.

Applications

  • HCP coverage evaluations
  • Western Blot (WB)
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Research use in process development and HCP monitoring for E. coli-derived products

Kit Contents

Anti-E.coli (K-12 & Alkaline Lysis) HCP Antibody, 1 mg2-8 °C (short-term); < -65 °C (long-term)
Storage buffer: PBS2-8 °C
* Total: N/A (antibody reagent) | Method: ELISA / Western Blot (antibody compatibility)

Attention

• For research use only. Not intended for diagnostic purposes.

• Maintain at 2-8 °C for short-term use; for long-term storage store below -65 °C.

• Do not freeze-thaw repeatedly; aliquot to avoid multiple freeze-thaw cycles.

• Contains no preservatives; handle under aseptic conditions where appropriate.

Quality Management & Certifications

Quality System

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QMS (ISO, GMP)

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Quality Advantages

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Quality Control Process

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📂 Technical Resources & Downloads

Frequently Asked Questions (FAQ)

Q1: What is the recommended storage condition for this antibody?
Store at 2-8 °C for short-term use. For long-term storage, keep below -65 °C. Product is stable for 12 months under recommended storage.
Q2: What applications is this antibody validated for?
This antibody is intended for HCP coverage evaluations and is suitable for use in Western blot and ELISA-based assays.
Q3: Is this product suitable for diagnostic use?
No. This product is provided for research use only (RUO) and is not intended for diagnostic purposes.
Q4: What is the immunogen used to generate this antibody?
The antibody was raised against E. coli host cell proteins (K-12 strains) produced chemically by alkaline lysis.

Research Use Only

Research Use Only (RUO) - Not for diagnostic use – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.

Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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