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For Research Use Only (RUO)
$1,010.00
Sku: 1104193
Product Description
SHENTEK® Animal-derived Biomaterial Residual DNA Sample Preparation Kit uses magnetic particle separation technology to extract residual DNA from a broad range of animal-derived biomaterials. The kit is designed for efficient and reproducible recovery of residual DNA from complex sample matrices including extracellular matrix, collagen and bio-absorbable membranes. Extracted DNA is compatible with downstream residual DNA quantitation by qPCR or PicoGreen assays. The kit supports manual sample preparation and automated extraction using the rHCDpurify system. Reagents are supplied for 50 extractions. Intended for research use only.
Technical Specifications
| Parameter | Details |
|---|---|
| Product number / Version | Product No.: 1104193; User Guide Version: A/0 |
| Intended use | Research Use Only (RUO) — residual DNA extraction from animal-derived biomaterials |
| Reactions / Extractions per kit | Reagents for 50 extractions |
| Overall kit shelf life | Up to 24 months (check label for expiration date) |
| Magnetic particles storage | 2-8 °C |
| Cold-stored reagents | Glycogen: -20 °C |
| Proteinase K digestion (per sample) | 20 µL Proteinase K + 100 µL Proteinase K Buffer + 80 µL DEPC-treated H2O |
| Proteinase K digestion conditions | Incubate at 55 °C for 60 minutes (dry bath); adjust amount/time for difficult samples |
| Binding buffer (per sample) | 200 µL Binding solution + 9 µL Glycogen + 0.2 µL Yeast tRNA (Do not add Yeast tRNA when using PicoGreen assay) |
| Binding step volumes | After adding binding buffer, add 200 µL isopropanol and 30 µL magnetic particles per sample |
| Wash volumes | Wash buffer A: 700 µL; Wash buffer B: 700 µL (per wash step) |
| Elution | Add 100–150 µL pre-warmed (70 °C) Elution buffer; incubate at 70 °C for 7 minutes, vortex intermittently |
| Magnetic separation time | Particle separation typically ~3–5 minutes on a magnetic stand |
| Automation compatibility | Compatible with rHCDpurify automated extraction (rHCD-193 program described) |
| Controls recommended | Negative control sample (NCS, 1×PBS) and sample extraction recovery control (ERC) recommended |
| Environmental conditions for instrument | Ambient temperature should not be lower than 22 °C for automated runs; UV sterilization recommended before/after runs |
Features
- Magnetic particle separation: Uses magnetic particle technology for efficient nucleic acid binding and purification from complex matrices.
- Optimized for animal-derived biomaterials: Validated for complex matrices such as extracellular matrix, collagen and bio-absorbable membranes.
- Compatibility with downstream assays: Extracted DNA is suitable for qPCR quantitation and PicoGreen analysis.
- Flexible workflow: Supports manual extraction and high-throughput automated extraction on the rHCDpurify system.
- Included controls and reagents: Includes reagents and recommended controls (NCS, ERC) to assess recovery and contamination.
Applications
- Residual DNA quantitation in animal-derived biomaterials
- Quality control testing of collagen, extracellular matrix products and bio-absorbable membranes
- Preclinical / manufacturing residual DNA assessment
- Sample preparation for qPCR or fluorescence-based DNA assays (PicoGreen)
Kit Contents
Wash buffer A (NND015) 15 mL × 1 bottleRoom temperature (add 20 mL anhydrous ethanol before first use)
Binding solution (NND017) 10 mL × 1 bottleRoom temperature
Elution buffer (NND020) 20 mL × 1 bottleRoom temperature
1× PBS (NND042) 5 mL × 1 bottleRoom temperature
DEPC-treated H2O (NND045) 5 mL × 1 bottleRoom temperature
Proteinase K Buffer (NND025) 5 mL × 1 bottleRoom temperature (if cloudy, heat at 37 °C until clear)
Magnetic particles (NND030) 750 µL × 2 tubes2-8 °C
Proteinase K (NND023) 500 µL × 2 tubesRefer to label
Glycogen (NND035) 500 µL × 1 tube-20 °C
Yeast tRNA (NND037) 50 µL × 1 tubeRefer to label
* Total: 50 extractions | Method: Magnetic particle separation-based DNA extraction (manual or automated with rHCDpurify)
Attention
• Read MSDS and wear appropriate PPE (gloves, eyewear, mask, lab coat).
• Add 20 mL anhydrous ethanol to Wash buffer A before first use; prepare Wash buffer B as 70% ethanol with ddH2O.
• Prepare Proteinase K digestion solution freshly; incubate samples at 55 °C for 60 min (adjust as needed).
• Do not add Yeast tRNA when using PicoGreen DNA quantitation.
• Resuspend magnetic particles thoroughly before use; avoid over-drying pellets—dry 30 s to 3 min depending on conditions.
• Centrifuge after vortexing to collect liquid from tube cap and walls before magnetic separation.
• Transfer eluate immediately after elution and perform downstream assays the same day to ensure accuracy.
• Store magnetic particles at 2–8 °C; cold-stored reagents (e.g., glycogen) should be kept at -20 °C.
Quality Management & Certifications
Quality System
Download QMSQMS (ISO, GMP)
Download CertificateQuality Advantages
View ReportQuality Control Process
Download ProcessTechnical Resources & Downloads
Frequently Asked Questions (FAQ)
Q1: Which downstream assays are compatible with the extracted DNA?
Extracted DNA is compatible with qPCR-based residual DNA quantitation and fluorescence-based assays such as PicoGreen. Do not add Yeast tRNA if using PicoGreen.
Q2: What is the recommended digestion protocol for samples?
Prepare Proteinase K digestion solution (20 µL Proteinase K + 100 µL Proteinase K Buffer + 80 µL DEPC H2O per sample) and incubate at 55 °C for 60 minutes. Adjust enzyme amount and digestion time for difficult-to-digest or cross-linked samples.
Q3: How should Wash buffer A be prepared before first use?
Add 20 mL of anhydrous ethanol to Wash buffer A (NND015) before first use as instructed in the manual.
Q4: Can I run the kit on an automated platform?
Yes. The kit is compatible with the rHCDpurify system (program rHCD-193). Follow the plate layout and column reagent volumes specified in the manual and perform UV sterilization as recommended.
Research Use Only
✔
Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.
✔
Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
