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Sku: 1101107-1
Categories: KitQC Kits

Introduction

Components Shipping Condition Unit Size Product Line Detection Method
E. coli DNA Control -20℃ 100 Reactions SHENTEK™ Probe-based qPCR
qPCR Master MIX      
DNA Dilution Buffer (DDB)      
E. coli Primer&Probe MIX (Incl IPC) -20℃, protect from light      


Overview

SHENTEK™ Residual E. coli DNA Quantitation Kit is used to quantitate residual E. coli host cell DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit uses duplex real-time PCR technology to perform rapid, specific, and reliable quantitation assay at the fg level. IPC(Internal Positive Control) is included in the E. coli Primer&Probe MIX to evaluate the performance of each PCR reaction. For extraction information, please refer to the SHENTEK™ Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).

 

Specification

Range 3x10-2-3x102 pg/μL, R2=0.99968, AE=97.5%
Accuracy Recovery = 81.2%-107.4% , CV<30%
LOQ 0.03 pg/μL
LOD (LLOQ) 0.003 pg/μL
Precision 2.2%-17.9% (<30%)
Robustness Freeze-thaw stability over 5 cycles
Instrument suitability not limited to Thermo, Biorad, HZSKBio qPCR equipments


Related Products

SHENTEK™ Residual Host Cell DNA Sample Preparation Kit

 

Documents

User Guide

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

Terms and Conditions

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