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Description
The 5 mL Gravity Columns (total volume 6.5 mL) are economical, disposable tools designed for small-scale gravity flow protein and antibody purification, buffer exchange, and desalting. Supplied empty (no resin included), they feature durable polyethylene frits, clear graduations, and secure snap-on caps—offering flexibility, reliability, and cost-effective performance. Compact and easy to handle, these columns are ideal for rapid sample cleanup prior to SDS-PAGE, HPLC, or mass spectrometry.
Specification
| Package | 5/PK/25/PK |
| Full name | Gravity Columns, 5 ml |
| Applications | The resin bed volume is 2.5 ml.Supplied with caps and stoppers. |
| Summary | The 5ml empty, plastic, disposable columns have an internal volume of 6.5ml and is designed for small scale gravity flow purifications. |
| Features | Total Volume: 6.5ml Resin Volume: 2.5ml Reservoir Volume: 4ml Closure: Plastic Stopper Cap: Push in cap Frit: 1.5mm ~30μm hydrophobic polyethylene |
| Composition | ?Columns, matching cap, frits, and rubber stoppers. |
| Total Volume | 6.5 ml |
| Resin Volume | 2.5 ml |
| Reservoir Volume | 4 ml |
| Closure | Plastic Stopper |
| Cap | Snap-on cap |
| Frit | 1.5 mm ~30 μm hydrophobic polyethylene. |
| Component | ?Columns, matching cap, frits, and rubber stoppers. |
Gravity Columns, 5 mL – FAQ
Q: What are they used for?
A: Perfect for small-scale protein and antibody purification, buffer exchange, and quick sample cleanup before downstream analysis.
Q: What samples can I load?
A: Typically cell lysates, fermentation supernatants, or antibody-containing culture media.
Q: What quality matters most?
A: Secure snap-on caps, stable frits for uniform flow, and clear columns ensure reliable and reproducible results.
Q: Any common challenges?
A: Flow control and recovery can be tricky at very small scales, but our well-designed frits and column geometry minimize dead volume and sample loss.
Q: Why choose these columns?
A: They’re compact, clean, consistent, and cost-effective—with multiple pack sizes (5 pcs / 25 pcs) for flexible lab use.
Q: Do these columns fit LabMate-style racks?
A: Yes—our 5 mL gravity columns fit most LabMate-style and universal chromatography racks designed for ~12 mm wells. If your rack uses special-size wells, please check the well diameter before purchase.
Q: Can I collect into 1.5 mL, 2 mL, or 15 mL tubes?
A: Yes. The outlet geometry allows stable positioning over microcentrifuge tubes (1.5/2 mL) as well as 15 mL conical tubes.
Q: Are these compatible with ion-exchange/affinity/gel-filtration resins?
A: Yes. The columns are supplied empty (no resin) and are compatible with common IEX, affinity (Ni-NTA, Protein A/G, GST, etc.), and size-exclusion media.
Storage
Room temperature
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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