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Description
Stain Free Bis-Tris Precast Gels are a safe, fast, and high-performance precast polyacrylamide gel designed for protein separation. The protein bands on this gel can be directly imaged under UV light, eliminating the need for staining. Our precast gel is available in 12-well or 15-well formats, with recommended maximum sample volumes of 35 μL and 25 μL, respectively. The dimensions are as follows:
Gel plate: 100 × 85 × 4.7 mm
Gel: 85 × 70 × 1 mm
Additionally, the Tris/MOPS/SDS electrophoresis buffer provided with the gel is neutral, enhancing gel stability and preventing protein re-modification during electrophoresis.
This product can be transported at room temperature, but not at temperatures lower than 0°C. It should be stored at 4°C, while the catalyst should be stored at -20°C after being suspended in pure water. The shelf life is 12 months when stored at 4–25°C. The shelf life is 8 months when stored at 4°C. Do not freeze Stain Free Bis-Tris Precast Gels. Using expired gels or improperly stored gels may result in poor band resolution.
Features
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Colorful Stacking Gels:
Easy sample loading.
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Neutral pH:
Resulting in sharper band resolution and accurate results.
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Stain Free:
Protein bands can be observed after electrophoresis and transfer without additional staining.
Non-migratory
The dyes do not migrate during electrophoresis.
The dyes do not diffuse or fade during gel staining and destaining.
Stain Free
Gels enable the direct visualization of protein bands under UV light both before and after transfer to a membrane.
1A: Stain-free visualization of proteins in gel, imaging at UV 302nm, taken by mobile phone;
1B: Stain-free visualization of proteins in gel with stain-free enabled imaging systems;
2: UV images of A. post-transfer PVDF membrane; B. protein gel.
Specifications
| For Use With (Equipment) | Mel Percentage |
| Gel Percentage | 12% |
| Gel Thickness | 1.0 mm |
| Gel Type | Bis-Tris |
| Sample Loading Volume | Up to 25 / 35 μL corresponding to 15-well |
| Separation Range | 10 to 250 kDa |
| Shelf Life | 12 Months |
| Wells | 15-well |
| Quantity | 10 / 20 Gels/Box |
| Shipping Condition | 4–25°C or Room Temperature |
| Storage Requirements | Store at 2-8°C. Do not freeze |
Storage
Transport at room temperature and Store at 4°C. Do not freeze.
Documents
INFO
Q&A
1. Are these precast gels compatible with the Invitrogen Xblot and Bio-Rad Mini Trans-Blot systems?
A: Yes, Biofargo’s precast gels are fully compatible with the Bio-Rad Mini Trans-Blot system.
For the Invitrogen Xblot system, we are in the process of confirming compatibility.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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