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$470.00
Sku: BF-56560001
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by September 25, 2026
Description
The Crithidia fasciculata Probe qPCR Kit is designed for the qualitative detection of Crithidia fasciculata DNA using probe-based real-time PCR. Purified DNA is amplified with target-specific primers and a fluorescent probe for real-time monitoring.
The kit supports laboratory research involving cultured organisms, kinetoplast DNA, kinetoplastid biology, and insect–protozoan interactions.
Taxonomic note: Crithidia luciliae remains listed separately in some databases and culture collection records. Molecular research has associated the ATCC 30258 culture with Crithidia fasciculata. Detection coverage depends on the validated primer-probe design.
Product note: The standard kit is supplied without an internal control.
Specifications
| Product Name | Crithidia fasciculata Probe qPCR Kit |
|---|---|
| Target Organism | Crithidia fasciculata Léger |
| Related Taxonomic Name | Crithidia luciliae (Strickland) Wallace and Clark |
| Target Material | Extracted DNA |
| Detection Method | Probe-based real-time PCR |
| Detection Type | Qualitative detection |
| Pack Size | 50 reactions |
| Internal Control | Not included |
| NCBI Taxonomy ID | 5656 |
| Intended Use | Laboratory research use only |
Features
- Qualitative detection of Crithidia fasciculata DNA.
- Target-specific probe-based qPCR format.
- Suitable for purified research DNA samples.
- Includes a non-infectious plasmid-based positive control.
- Supplied for 50 reactions.
- For laboratory research use only.
Kit Components
| Component | Description | Size | Lid Color |
|---|---|---|---|
| 2× Probe qPCR Master Mix | Optimized buffer and enzyme mixture | 500 µL | Blue |
| Primer-Probe Powder | Lyophilized target-specific primer and probe set | 50 reactions | Brown |
| Positive Control | Non-infectious plasmid-based control | 0.5 mL | Yellow |
| Nuclease-Free Water | For reaction preparation and dilution | 1 mL | Green |
Applications
- Qualitative detection of Crithidia fasciculata DNA.
- Molecular confirmation of laboratory cultures.
- Kinetoplast and trypanosomatid molecular biology research.
- Insect–protozoan interaction studies.
Use extracted DNA of suitable purity. Applicable sample types depend on the validated extraction method and laboratory workflow.
Storage & Shipping
- Store at −20°C.
- Ship under dry-ice conditions.
- Avoid repeated freeze–thaw cycles.
- Protect fluorescent probe components from prolonged light exposure.
FAQ
The kit is designed for the qualitative detection of Crithidia fasciculata DNA using probe-based real-time PCR.
Molecular studies have associated the ATCC 30258 culture listed as Crithidia luciliae with Crithidia fasciculata. Detection coverage should be confirmed from the validated primer-probe design.
No. The standard kit is supplied without an internal control.
No. This product is intended strictly for laboratory research use.
For Research Use Only. Not for use in diagnostic procedures.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
