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$3,200.00
Sku: D-6080IM.RFP
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by August 18, 2026
Description
Primary canine vascular smooth muscle cells have a limited culture lifespan, while unlabeled cells can be difficult to distinguish in fluorescence imaging and cell-visualization workflows. Immortalized RFP-Expressing Canine Primary Aortic Smooth Muscle Cells provide an extended-lifespan, fluorescently labeled cell model derived from the aorta tissue of beagle dogs.
The canine aortic smooth muscle cells are grown in gelatin pre-coated tissue culture flasks with Complete Growth Medium. Cells are harvested at passages 2–3, cryopreserved, and supplied frozen at a concentration of 0.5 × 106 cells/mL.
RFP expression is introduced by transduction with RFP-lentiviral particles. Red fluorescent protein produces bright red fluorescence when exposed to yellow-green light, supporting workflows that require visualization of RFP-labeled canine smooth muscle cells.
The cells are characterized by immunofluorescence staining with an α-smooth muscle actin antibody and are reported negative for bacteria, yeast, fungi, and mycoplasma. Under the specified culture conditions, these immortalized canine aortic smooth muscle cells can be expanded for more than 10 passages using a 1:2 split ratio.
Catalog No. D-6080IM.RFP. For research use only.
Specifications
| Product Name | Immortalized RFP-Expressing Canine Primary Aortic Smooth Muscle Cells |
|---|---|
| Catalog No. | D-6080IM.RFP |
| Species | Canine |
| Donor Breed | Beagle dog |
| Tissue Source | Aorta tissue |
| Cell Type | Immortalized canine aortic smooth muscle cells derived from primary cells |
| Fluorescent Label | Red fluorescent protein (RFP) |
| RFP Introduction | Transduction with RFP-lentiviral particles |
| Passage at Cryopreservation | Passages 2–3 |
| Cell Concentration | 0.5 × 106 cells/mL |
| Product Format | Cryopreserved frozen vial |
| Characterization | Immunofluorescence staining with an α-smooth muscle actin antibody |
| Expansion | More than 10 passages under the specified culture conditions |
| Recommended Split Ratio | 1:2 |
| Culture Surface | Gelatin pre-coated tissue culture flasks |
| Suggested Medium | M2268 Complete Smooth Muscle Cell Medium w/ Kit – 500 mL |
| Microbial Testing | Negative for bacteria, yeast, fungi, and mycoplasma |
| Shipping | Shipped frozen with dry ice overnight |
| Long-Term Storage | Liquid nitrogen at approximately −180°C immediately upon arrival |
| Intended Use | For research use only |
Features
- Immortalized RFP-expressing canine aortic smooth muscle cells derived from beagle dog aorta tissue
- RFP introduced through transduction with RFP-lentiviral particles
- Derived from primary canine aortic smooth muscle cells with an extended culture lifespan
- Harvested and cryopreserved at passages 2–3
- Supplied frozen at a concentration of 0.5 × 106 cells/mL
- Characterized by α-smooth muscle actin immunofluorescence staining
- Reported negative for bacteria, yeast, fungi, and mycoplasma
- Expandable for more than 10 passages at a 1:2 split ratio under specified conditions
- Recommended for culture in gelatin pre-coated tissue culture flasks
- Shipped frozen on dry ice for immediate transfer to liquid nitrogen storage
Applications
The combination of canine aortic smooth muscle origin, extended culture lifespan, and introduced RFP expression may support research workflows involving:
- Canine vascular smooth muscle cell research
- Canine aortic smooth muscle cell culture studies
- Fluorescence imaging of RFP-labeled canine cells
- RFP-based cell visualization and in vitro cell tracking
- Comparison of fluorescently labeled and unlabeled canine vascular cells
- Passage-related studies using an immortalized canine smooth muscle cell line
- Development of in vitro canine vascular smooth muscle research models
Suitability for a specific assay or experimental system should be determined and validated by the investigator. Functional performance in disease modeling, compound screening, migration, contraction, differentiation, or co-culture assays is not specified in the supplied product documentation.
RFP Labeling and Cell Identity
RFP-expressing cells are developed through transduction with RFP-lentiviral particles. Red fluorescent protein produces bright red fluorescence when exposed to yellow-green light, providing a fluorescent label for cell visualization.
Cell identity is characterized by immunofluorescence staining with an α-smooth muscle actin antibody. The supplied documentation does not state an RFP-positive percentage, fluorescence intensity value, passage-specific fluorescence stability result, or instrument-specific excitation and emission settings.
Culture Requirements
- Use M2268 Complete Smooth Muscle Cell Medium w/ Kit – 500 mL as the suggested medium.
- Culture the cells in gelatin pre-coated tissue culture flasks.
- Under the specified culture conditions, use a recommended split ratio of 1:2.
- The cells can be expanded for more than 10 passages when cultured under the specified conditions.
- Repeated freezing and thawing are not recommended.
Follow the applicable cell culture protocol and laboratory aseptic procedures. Specific incubation conditions, seeding density, coating concentration, medium-change schedule, and subculture reagents are not stated in the supplied product datasheet.
Quality Control
| Quality Attribute | Documented Result |
|---|---|
| Cell Identity Characterization | Immunofluorescence staining with an α-smooth muscle actin antibody |
| Bacteria | Negative |
| Yeast | Negative |
| Fungi | Negative |
| Mycoplasma | Negative |
Shipping and Storage
- Cryopreserved cells are shipped frozen with dry ice overnight.
- Upon arrival, immediately transfer the frozen vial to liquid nitrogen storage at approximately −180°C until ready for use.
- Do not use a −20°C or −80°C freezer for routine long-term storage of the frozen cells.
- Repeated freezing and thawing are not recommended.
- Live-cell shipment may be available upon request.
Research Use and Handling
- For research use only.
- Not authorized for human use, in vitro diagnostic use, or therapeutic procedures.
- Handle all animal cells with caution and treat them as potential pathogens because no testing procedure can completely guarantee the absence of infectious agents.
- Transfer or resale may be subject to supplier terms and written authorization.
Documents
D-6080IM.RFP Product Datasheet
Frequently Asked Questions
What are Immortalized RFP-Expressing Canine Primary Aortic Smooth Muscle Cells?
They are extended-lifespan canine aortic smooth muscle cells derived from the aorta tissue of beagle dogs. RFP is introduced through transduction with RFP-lentiviral particles, and the cells are supplied as a cryopreserved frozen vial.
Are these primary cells or an immortalized canine aortic smooth muscle cell line?
The cells are derived from primary canine aortic smooth muscle cells and have undergone immortalization to extend their culture lifespan. The supplied product is therefore an immortalized cell model rather than an unmodified, finite-lifespan primary cell preparation.
What tissue and animal breed are the cells derived from?
The cells are derived from aorta tissue collected from beagle dogs.
How is RFP introduced into the canine aortic smooth muscle cells?
According to the product datasheet, RFP-expressing cells are developed by transduction with RFP-lentiviral particles.
Is the specific immortalization method identified for D-6080IM.RFP?
The product documentation describes the immortalization platform as overexpression of either simian virus 40 large T antigen (SV40) or human telomerase reverse transcriptase (hTERT). It does not identify which of these two methods was used specifically for D-6080IM.RFP.
At what passage are the cells cryopreserved?
The cells are harvested and cryopreserved at passages 2–3.
What cell concentration is supplied?
The datasheet states that each cryopreserved vial contains cells at a concentration of 0.5 × 106 cells/mL. The document does not specify the vial fill volume or a separate guaranteed total cell count per vial.
How are the canine aortic smooth muscle cells characterized?
The cells are characterized by immunofluorescence staining with an α-smooth muscle actin antibody.
What quality control testing is documented?
The product datasheet reports negative results for bacteria, yeast, fungi, and mycoplasma.
How many passages can the immortalized cells be expanded?
Under the specified culture conditions, the cells can be expanded for more than 10 passages using a recommended split ratio of 1:2.
What medium and culture surface are recommended?
The suggested medium is M2268 Complete Smooth Muscle Cell Medium w/ Kit – 500 mL. The cells are grown in gelatin pre-coated tissue culture flasks.
Can the RFP-expressing cells be used for fluorescence imaging or cell tracking?
The introduced red fluorescent protein provides a fluorescent label that may support RFP-based cell visualization, fluorescence imaging, and in vitro cell-tracking workflows. Investigators should validate compatibility with their imaging system and specific experimental design.
Does the datasheet provide an RFP-positive rate or fluorescence stability result?
No. The supplied datasheet does not state an RFP-positive percentage, quantitative fluorescence intensity, or passage-specific fluorescence stability result.
How are the frozen cells shipped and stored?
The cryopreserved cells are shipped frozen with dry ice overnight. Upon arrival, transfer the vial immediately to liquid nitrogen at approximately −180°C until ready for use.
Can the cells be stored in a −20°C or −80°C freezer?
No. The product documentation states that the frozen cells should not be kept in a −20°C or −80°C freezer for routine storage. Long-term storage requires liquid nitrogen.
Can the cells be refrozen after thawing?
Repeated freezing and thawing are not recommended.
Are these cells intended for clinical, diagnostic, or therapeutic use?
No. D-6080IM.RFP is for research use only and is not authorized for human use, in vitro diagnostic procedures, or therapeutic procedures.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
