Precast-Gel Hepes-Tris SDS-PAGE 4-15% 10Well

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Sku: P310061-10 PCS
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Description

Precast-GLgel Hepes SDS-PAGE 4–15% (10-Well) is a ready-to-use gradient gel designed for efficient and high-resolution protein electrophoresis. Using glass plate construction, it minimizes non-specific adsorption and produces sharper, clearer protein bands.
This 4–15% HEPES-based gradient gel offers excellent separation performance for medium to large molecular weight proteins, and provides gentler running conditions suitable for membrane proteins and protein complexes. Pre-cast and supplied with running buffer, it eliminates the need for manual gel preparation, ensuring convenience, time savings, and better reproducibility across experiments.

Specification

Full name Precast-GLgel Hepes SDS-PAGE 4–15% 10-Well
Package 10 pcs
Components 10 pcs gel, 10 pcs SDS-PAGE running buffer
Gel size 8 × 7.4 × 0.15 cm
Plate size 9.8 × 8.4 × 0.41 cm
Wells 10 wells
Buffer system HEPES buffer system
Maximum sample loading volume 60 µL
Recommended sample loading volume 30 µL

 

Buffer

Buffer Hepes Running Buffer for SDS-PAGE (Powder)
Specification 10*500 mL
Introduction Hepes Running buffer is formulated for separation of proteins in their denaturing or nondenaturing state on Hepes-Tris gels. This product is available in powder form, a total of 10 packs, each pack can prepare 500 mL of 1× working solution.
Protocol (for reference only)
  • Take 1 pack of powder that can prepare 500 mL of hepes running buffer, pour it into a clean beaker, add 400 mL distilled water and stir to dissolve the powder completely.
  • Make up to 500 mL in the graduated cylinder with distilled water, which is 1× working solution and can be used after mixing.
  • Unused running buffer can be stored at room temperature, usually 2-3 days at room temperature and 1-2 weeks at 4°C.
Notes
  • 1× working buffer should not be used for more than 2 weeks after preparation.
  • This running buffer is suitable for Hepes-Tris gels, and should not be used for gels with other chemistries.
  • For research use only. Not for use in diagnostic procedures.
  • For your safety and health, please wear personal protective equipment and clothing when operating.
Storage Store at room temperature, valid for 2 years.

 

Features

  • Glass plate design minimizes non-specific protein adsorption and produces sharper, clearer bands.

  • Automated gel casting ensures high consistency, stability, and reproducibility across batches.

  • 4–15% gradient formulation provides excellent separation for medium to high molecular weight proteins.

  • Suitable for membrane proteins and protein complexes, offering gentle running conditions provided by the HEPES buffer system.

  • SDS-free gel matrix supports both denaturing (SDS-PAGE) and non-denaturing (Native-PAGE) applications.

  • Ready-to-use format eliminates manual gel preparation, saving time and reducing procedural variability.

  • Long shelf life and easy storage at 2–8°C.

Applications

  • SDS-PAGE analysis of general proteins

  • Separation of medium to large molecular weight proteins

  • Electrophoresis of membrane proteins and membrane protein complexes

  • Native-PAGE for non-denaturing protein studies

  • Western blot (WB) sample preparation and protein detection

  • Protein analysis workflows requiring a 4–15% gradient gel

Storage

2~8°C, do not freeze

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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