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$5.00
Sku: P310138-200U
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by July 14, 2026
Description
Taq DNA Polymerase for PCR amplification is a recombinant thermostable DNA polymerase widely used in routine molecular biology experiments.
Taq DNA Polymerase is a recombinant DNA polymerase derived from a gene encoding Thermus aquaticus DNA polymerase and expressed in E. coli after plasmid cloning and induction. It is widely used as a standard PCR enzyme for DNA amplification in routine molecular biology workflows.
This enzyme lacks 3’→5’ exonuclease proofreading activity and exhibits very low 5’→3’ exonuclease activity, making it suitable for conventional PCR applications where high amplification efficiency is required.
Recombinant Taq DNA Polymerase is recommended for most PCR reactions and shows a half-life of > 40 min at 95°C. It provides stable performance for DNA amplification with a reported mutation rate of < 2.2×10⁻⁵ per cycle.
The enzyme is free from contamination of foreign nucleases and bacterial DNA, ensuring reliable amplification results. It is suitable for users requiring a stable and efficient Taq DNA polymerase for PCR amplification in routine laboratory applications.
Specification
| Parameter | Specification |
|---|---|
| Package | 1 KU/5 KU/200 U |
| Full name | Taq DNA Polymerase |
| Composition | Taq DNA polymerase (5 U/ul), 10X PCR Buffer without Mgcl2, 25 mM MgCl2 . |
| Reaction Buffer | 10X PCR Buffer |
| Unit Definition | One unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol deoxyribonucleotides into a polynucleotide fraction in 30 min at 74°C. |
| Concentration | 5 U/μl |
| Storage Buffer | 20 mM Tris-HCl (PH 8.0) ；0.1 mM EDTA,；1 mM DTT；0.5% Tween 20；100 mM KCl；0.5% NP-40；50% glycerin. |
| Component | Taq DNA polymerase (5 U/ul), 10X PCR Buffer without Mgcl2, 25 mM MgCl2 . |
Features
There is no contamination of foreign nuclease and bacterial DNA, and the amplification efficiency is high and the stability is good.
Applications
Taq DNA Polymerase is a thermostable enzyme widely used in routine molecular biology workflows for DNA amplification. It is suitable for a variety of PCR-based applications as described below:
-
Standard PCR amplification
Amplification of DNA fragments for downstream applications such as sequencing, cloning, and gel analysis. -
RT-PCR (after cDNA synthesis)
Used for amplification of cDNA templates derived from RNA in gene expression and transcript analysis studies. -
TA cloning applications
PCR products generated by Taq DNA Polymerase typically carry 3'-A overhangs, making them suitable for TA cloning vectors. -
Genotyping and mutation detection
Applied in SNP analysis, genetic screening, and routine mutation detection workflows. -
Routine and educational PCR use
Commonly used in teaching laboratories and routine research due to its robustness and cost-effectiveness.
Storage
-20°C
FAQ
What is Taq DNA Polymerase used for?
Taq DNA Polymerase is commonly used for PCR amplification of DNA. It enables the synthesis of DNA fragments for applications such as cloning, sequencing, and genetic analysis.
Why is Taq DNA Polymerase thermostable?
Taq DNA Polymerase is derived from the thermophilic bacterium Thermus aquaticus, allowing it to remain stable and functional at high temperatures used during PCR cycling.
Does Taq DNA Polymerase have proofreading activity?
No, Taq DNA Polymerase lacks 3’→5’ exonuclease proofreading activity, which results in a higher error rate compared to high-fidelity DNA polymerases.
What is the typical error rate of Taq DNA Polymerase?
The error rate is generally around <2.2 × 10⁻⁵ per nucleotide per cycle, depending on reaction conditions.
Can Taq DNA Polymerase be used for cloning?
Yes. PCR products generated by Taq DNA Polymerase often carry 3'-A overhangs, which are suitable for TA cloning strategies.
What is the optimal temperature for Taq DNA Polymerase activity?
The optimal extension temperature is approximately 72–74°C.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
