0 item(s)
View cart
You have no items in your shopping cart.
RUO Grade- Active- His-tag
$220.00
Sku: TL-636-0050
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by September 14, 2026
Description
Recombinant Human EPO Protein is a biologically active, glycosylated human erythropoietin produced in HEK293 cells. The recombinant protein is based on the human EPO reference sequence NP_000790.2 and contains a C-terminal His tag.
Erythropoietin is a glycoprotein cytokine that promotes the proliferation and differentiation of erythroid progenitor cells, including colony-forming unit-erythroid (CFU-E) cells, into mature erythrocytes. Physiological EPO production occurs primarily in the kidneys in adults, while the liver is the predominant source during fetal and neonatal development.
In addition to its established role in erythropoiesis, EPO has been studied for non-hematopoietic biological functions associated with angiogenesis, vascular smooth muscle cell proliferation, hypoxia-related neuroprotection, and the regulation of certain B-cell responses.
This HEK293-derived protein is validated in a TF-1 cell proliferation assay and is intended for in vitro cell culture and laboratory research involving erythropoiesis, EPO receptor signaling, and related biological processes. It is not intended for direct clinical or therapeutic use.
Specifications
| Specification | Acceptance Criteria | TL-636-0050 | TL-636-0100 |
|---|---|---|---|
| Package Size | Specified fill quantity | 50 µg | 100 µg |
| Expression Host | HEK293 cells | Compliance | Compliance |
| Reference Sequence | Human EPO, NP_000790.2 | Compliance | Compliance |
| Tag | C-terminal His tag | Compliance | Compliance |
| Predicted Molecular Weight | 19.2 kDa | Compliance | Compliance |
| Purification Method | Chromatographic purification | Compliance | Compliance |
| Appearance | White, loose lyophilized preparation | Compliance | Compliance |
| Identity | Electrophoretic band consistent with the expected molecular weight | Compliance | Compliance |
| Visible Particles | No visible particles | Compliance | Compliance |
| Moisture | ≤4.0% | 2.0% | 2.9% |
| pH | 6.5–7.5 | 7.3 | 7.3 |
| Purity | >90% by SDS-PAGE | Compliance | Compliance |
| Endotoxin | <0.1 EU/µg | <0.1 EU/µg | <0.1 EU/µg |
| Mycoplasma | Negative | Negative | Negative |
| Sterility | No growth | No growth | No growth |
| Biological Activity | TF-1 cell proliferation assay; specific activity ≥1.0×106 IU/mg | 3.08×106 IU/mg | Refer to lot-specific COA |
Features
-
✔
HEK293 Mammalian Expression
Produced in HEK293 cells to support mammalian protein folding and glycosylation.
-
✔
Validated Biological Activity
Evaluated using a TF-1 cell proliferation assay with a product specification of ≥1.0×106 IU/mg.
-
✔
Defined Protein Construction
Based on human EPO reference sequence NP_000790.2 with a C-terminal His tag and a predicted molecular weight of 19.2 kDa.
-
✔
Comprehensive Quality Control
Tested for identity, purity, endotoxin, moisture, pH, mycoplasma, sterility, and biological activity.
Applications
Erythropoiesis Research
Studies of the proliferation and differentiation of erythroid progenitor cells, including CFU-E cells.
EPO/EPOR Signaling Research
Investigation of erythropoietin receptor activation and EPO-associated cellular signaling pathways.
TF-1 Cell-Based Assays
Suitable for in vitro research using EPO-responsive TF-1 cell proliferation systems.
Non-Hematopoietic EPO Research
Research into reported EPO-associated functions involving angiogenesis, hypoxia responses, neuroprotection, and B-cell biology.
Stability & Storage
- Lyophilized Protein: Store at −20°C. The stated shelf life is 24 months when stored under the recommended conditions.
- Reconstitution: Reconstitute with sterile water, 0.9% NaCl, suitable cell culture medium, or PBS. A final concentration of at least 100 µg/mL is recommended.
- After Reconstitution: Aliquot and store at −20°C for up to 6 months or at −80°C for up to 12 months under appropriate sterile conditions.
- Handling: Avoid repeated freeze–thaw cycles. Refer to the lot-specific COA and current product label for applicable storage information.
Cautions
• Research Use Only: This product is intended for in vitro cell culture and laboratory research only. It must not be used directly for clinical treatment.
• Handling: Prepare solutions using aseptic technique and avoid microbial contamination to maintain protein activity.
• Storage: Store the lyophilized product at −20°C. After reconstitution, aliquot the solution and avoid repeated freeze–thaw cycles.
Documents
FAQ
What is Recombinant Human EPO Protein?
Recombinant Human EPO Protein is a biologically active, glycosylated form of human erythropoietin produced in HEK293 cells. It promotes the proliferation and differentiation of erythroid progenitor cells and is supplied for in vitro cell culture and laboratory research.
How is Recombinant Human EPO Protein produced?
The protein is produced using a HEK293 mammalian expression system. It is based on the human EPO reference sequence NP_000790.2 and contains a C-terminal His tag.
What is the predicted molecular weight of this protein?
The predicted molecular weight is 19.2 kDa. Because EPO is a glycosylated protein, its apparent molecular weight observed by SDS-PAGE may differ from the sequence-based predicted value.
What applications can Recombinant Human EPO Protein be used for?
The protein is suitable for in vitro research involving erythropoiesis, erythroid progenitor cell differentiation, EPO receptor signaling, TF-1 cell proliferation, and other EPO-associated biological processes.
How is the biological activity of Recombinant Human EPO Protein validated?
Biological activity is evaluated using a TF-1 cell proliferation assay. The product specification requires a specific activity of ≥1.0×106 IU/mg. Refer to the lot-specific Certificate of Analysis for the measured activity of the supplied lot.
What quality controls are performed?
Quality testing includes appearance, identity, visible particle inspection, moisture, pH, purity by SDS-PAGE, endotoxin, mycoplasma, sterility, and biological activity.
The product specification includes purity greater than 90% by SDS-PAGE and endotoxin below 0.1 EU/µg.
How should the lyophilized protein be stored?
Store the lyophilized protein at −20°C. The stated shelf life is 24 months when the product is maintained under the recommended storage conditions.
How should the protein be reconstituted and stored afterward?
Reconstitute with sterile water, 0.9% NaCl, suitable cell culture medium, or PBS at a final concentration of at least 100 µg/mL. After reconstitution, aliquot and store at −20°C for up to 6 months or at −80°C for up to 12 months. Avoid repeated freeze–thaw cycles.
Is Recombinant Human EPO Protein intended for therapeutic or clinical use?
No. This product is intended for in vitro cell culture and laboratory research only. It must not be used directly for human or animal clinical treatment, diagnostic procedures, or therapeutic applications.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
