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$103.00
Sku: Y07901B-10ug
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by July 29, 2026
Description
Recombinant Bovine Transforming Growth Factor Beta 3 (TGF-β3) is a multifunctional cytokine that plays a key role in regulating cell proliferation, differentiation, migration, and immune responses. As a member of the TGF-β superfamily, TGF-β3 is involved in embryonic development, tissue regeneration, and extracellular matrix production. It signals through TGF-β receptors to activate SMAD-dependent and independent pathways.
This recombinant protein is expressed in CHO cells and corresponds to the mature region of bovine TGF-β3 (Arg337–Ser452), ensuring proper folding and biological activity. With high purity (≥95%) and strong bioactivity (ED50 ≤0.3 ng/mL in TF-1 cell inhibition assay), this product is suitable for research in cell biology, immunology, and tissue engineering. The protein is supplied in a tag-free, lyophilized form for convenient handling and storage.
Specifications
| Product Details | |
|---|---|
| Cat. No. | Y07901 |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤10 EU/mg by the LAL method. |
| Bioactivity | Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.3 ng/mL. |
| Accession | A6QP91 |
| Alternative Names | Transforming growth factor beta-3; TGFB3; TGF-beta-3; Latency-associated peptide; LAP |
| Species | Bovine |
| Expression System | CHO |
| Mol. Weight | 13 kDa |
| Expression Region | Arg337-Ser452 |
| Tag Info | Tag free |
| Formulation | Lyophilized |
| Buffer | Lyophilized from a 0.22-μm filtered solution containing 100 mM Glycine, 150 mM NaCl, 5% mannitol and 0.01% Tween 80, pH 4.0. |
| Size | 10 μg / 50 μg / 1 mg / 20 mg |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution; Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring reliable and reproducible experimental results.
- ✔ High Biological Activity: Exhibits strong inhibitory activity with an ED50 ≤0.3 ng/mL in TF-1 cell-based assay, ensuring consistent performance.
- ✔ Mammalian Expression System: Produced in CHO cells to ensure correct protein folding and native-like post-translational modifications.
- ✔ Stable Formulation: Lyophilized with optimized buffer system to maintain protein stability during storage and transport.
Applications
Tissue Engineering
Involved in extracellular matrix production and tissue repair, widely used in regeneration studies.
Immunology
Modulates immune responses and plays a role in immune suppression and inflammation regulation.
Cell Biology
Regulates cell proliferation, differentiation, and apoptosis via TGF-β signaling pathways.
Data Figures
Figure 1: Measured in a inhibition assay using TF-1 cells in the presence of IL4.
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
FAQ
What is Recombinant Bovine TGF-beta 3 Protein?
Recombinant Bovine TGF-beta 3 Protein is a recombinant form of bovine Transforming Growth Factor Beta 3 (TGF-β3). This protein corresponds to the mature region of bovine TGF-β3 (Arg337-Ser452) and is produced using a Chinese Hamster Ovary (CHO) cell expression system.
What expression system is used for this Bovine TGF-β3 Protein?
This recombinant Bovine TGF-β3 Protein is expressed using a Chinese Hamster Ovary (CHO) cell line. The product is supplied as a recombinant cytokine protein for research applications.
What are the purity and bioactivity specifications of this recombinant TGF-beta 3 protein?
The recombinant TGF-beta 3 protein has a purity of ≥95% determined by SDS-PAGE with Coomassie staining. Its biological activity is measured using a TF-1 cell inhibition assay in the presence of IL-4, with an ED50 value of ≤0.3 ng/mL.
What is the molecular weight of Recombinant Bovine TGF-β3 Protein?
The predicted molecular mass of Recombinant Bovine TGF-β3 Protein is approximately 13 kDa.
How is Recombinant Bovine TGF-beta 3 Protein supplied?
This Bovine TGF-β3 Protein is supplied in lyophilized form. The product is lyophilized from a 0.2 μm filtered solution containing glycine, sodium chloride, mannitol, and Tween 80 at pH 4.0.
How should Recombinant TGF-beta 3 Protein be reconstituted?
It is recommended to redissolve the lyophilized Recombinant Bovine TGF-beta 3 Protein in sterile deionized water before use.
How should Bovine TGF-β3 Protein be stored?
The lyophilized protein can be stored at -20°C to -80°C for 36 months. After reconstitution, the product can be stored for 6 months at -20°C to -80°C under sterile conditions, or 7-10 days at 2°C to 8°C under sterile conditions. Repeated freeze-thaw cycles should be avoided.
What research applications is Recombinant Bovine TGF-beta 3 Protein suitable for?
Recombinant Bovine TGF-beta 3 Protein is suitable for research involving TGF-beta 3 cytokine studies, recombinant cytokine protein experiments, and cell-based research workflows requiring bovine TGF-β3 protein.
Is Recombinant Bovine TGF-beta 3 Protein for clinical use?
No. This product is intended for Research Use Only (RUO) and is not intended for diagnostic, therapeutic, or clinical applications.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
