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$129.00
Sku: Y07201-10ug
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by June 06, 2026
Description
Recombinant Human Thrombopoietin (TPO) is a key hematopoietic growth factor that regulates the production, differentiation, and maturation of megakaryocytes and platelets. It exerts its biological effects by binding to the c-Mpl receptor, activating downstream signaling pathways such as JAK/STAT, MAPK, and PI3K/AKT, which are essential for hematopoietic stem cell maintenance and platelet formation.
This recombinant protein is expressed in CHO cells and corresponds to the functional region of human TPO (Ser22–Leu195), ensuring proper folding and biological activity. With high purity (≥95%) and strong bioactivity (ED50 ≤1.0 ng/mL in Mo7e cell proliferation assay), this product is suitable for research in hematology, stem cell biology, and cytokine signaling. The protein is supplied in a tag-free, lyophilized form for convenient storage and reconstitution.
Specifications
| Product Details | |
|---|---|
| Cat. No. | Y07201 |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
| Endotoxin | ≤10 EU/mg by the LAL method |
| Bioactivity | Measured in a cell proliferation assay using Mo7e human leukemia cell line. The ED50 for this effect is ≤1.0 ng/mL. |
| Accession | P40225 |
| Alternative Names | rHuThrombopoietin/TPO; TPO |
| Species | Human |
| Expression System | CHO |
| Mol. Weight | 18.6 kDa |
| Expression Region | Ser22-Leu195 |
| Tag Info | Tag free |
| Formulation | Lyophilized |
| Buffer | PBS,5% mannitol and 0.01% Tween 80, pH7.4 |
| Size | 10μg/50μg/1mg |
| Lead Time | Products are in stock,but custom products may require further consultation |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring reliable and reproducible experimental results.
- ✔ High Biological Activity: Exhibits potent activity with ED50 ≤1.0 ng/mL in Mo7e cell proliferation assay, ensuring consistent performance.
- ✔ Mammalian Expression Systemy: Expressed in CHO cells to ensure correct folding and native-like biological functionality.
- ✔ Convenient and Reliable: Supplied in a tag-free, lyophilized form for easy reconstitution, stable storage, and compatibility with diverse experimental applications.
Applications
Hematology
Regulates megakaryocyte development and platelet production, widely used in hematopoietic research.
Stem Cell Research
Supports maintenance and expansion of hematopoietic stem and progenitor cells.
Cell Biology
Used to study cytokine signaling pathways such as JAK/STAT and mechanisms of cell proliferation and differentiation.
Data Figures
Figure 1: Measured in a cell proliferation assay using Mo7e cells. The ED50 for this effect is ≤1.0 ng/mL. The Bioactivity of Y07201 was higher than other competing products.
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
