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$127.00
Sku: TL-514-0010
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by June 06, 2026
Description
M-CSF is a disulfide-linked homodimeric glycoprotein and core member of the colony-stimulating factor family. By specifically binding the c-Fms receptor (CSF-1R/CD115), it induces receptor dimerization and internalization and activates PI3K-AKT/mTOR and Ras-MAPK signaling to drive hematopoietic stem cell differentiation toward monocyte/macrophage lineages while promoting osteoclast precursor proliferation and bone remodeling.
This protein exists as two bioactive isoforms: glycosylated and chondroitin sulfate-modified proteoglycan variants—the latter extends local bioactivity via extracellular matrix anchoring.
Immunologically, it mediates endothelial progenitor mobilization and neovascularization through macrophage-derived VEGF secretion while upregulating proinflammatory chemokines to potentiate innate immunity.
For cell therapy applications, it serves as a critical component in:
- ex vivo macrophage differentiation systems, synergizing with GM-CSF to induce M1-polarized anti-tumor macrophages for CAR-M therapies with enhanced tumor infiltration and phagocytosis;
- 3D bioscaffold cultures where spatiotemporal release kinetics engineer functional vascular networks to improve survival of tissue-engineered grafts.
Specifications
| Specification | Acceptance Criteria | TL-514-0010 | TL-514-0050 |
|---|---|---|---|
| Appearance | Freeze-dried preparation, the form should be white loose body | Compliance | Compliance |
| Identity | The electrophoretic bands should be consistent with the molecular weight of the product | Compliance | Compliance |
| Visible Particle | Should be no visible particle | Compliance | Compliance |
| Moisture | ≤4.0% | 0.9% | 0.6% |
| pH | 6.5~7.5 | 7.2 | 7.2 |
| Purity | >90.0% | Compliance | Compliance |
| Endotoxin | <0.1EU/μg | <0.1EU/μg | <0.1EU/μg |
| Mycoplasma | Should be negative | Negative | Negative |
| Sterility | No growth | No growth | No growth |
| Biological Activity | Cell proliferation assay was performed with M-NFS-60 cells, and the activity ≥1×107 IU/mg | 2.50×107 IU/mg | 3.88×107 IU/mg |
Features
-
✔
Native Dimeric M-CSF Activity
Disulfide-linked homodimer preserves authentic CSF-1R (c-Fms) activation and downstream signaling.
-
✔
High Biological Potency
Verified by M-NFS-60 cell proliferation assay with specific activity ≥1×107 IU/mg (lot values up to 3.88×107 IU/mg).
-
✔
Low Endotoxin, Stringent QC
90% purity with endotoxin <0.1 EU/µg; sterility and mycoplasma tested for sensitive cell culture use.
-
✔
Consistent Lot Performance
Tight control of moisture and pH supports reproducible differentiation outcomes.
Applications
Macrophage Differentiation Systems
Drives monocyte/macrophage lineage commitment for in vitro immune cell studies.
CAR-M & Tumor Immunology Research
Used alone or with GM-CSF to support macrophage activation and functional polarization.
3D Tissue Engineering & Vascularization
Facilitates macrophage-mediated angiogenic support in scaffold-based cultures.
Bone & Osteoclast Research
Supports studies on osteoclast precursor proliferation and bone remodeling pathways.
Stability & Storage
- Lyophilized powder: Stable for 12 months at −80 °C or 6 months at −20 °C when stored in the original sealed container under desiccant.
- Reconstitution: Dissolve in sterile Water for Injection, 0.9% NaCl, or PBS (pH 7.4), maintaining a final concentration ≥100 µg/mL.
- Handling: After reconstitution, aliquot and avoid repeated freeze–thaw cycles.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Prepare solutions using sterile technique. Avoid microbial contamination to maintain activity.
• Storage: Follow recommended storage conditions. Repeated freeze–thaw cycles may reduce performance.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
