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$32.00
Sku: Y03101-10ug
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by June 06, 2026
Description
Recombinant Human Insulin-like Growth Factor 1 (IGF-1) is a small peptide growth factor that plays a critical role in cell proliferation, differentiation, and survival. It is widely involved in growth and development processes and mediates many of the biological effects of growth hormone. IGF-1 binds to the IGF-1 receptor (IGF1R), activating downstream signaling pathways such as PI3K/AKT and MAPK, which regulate cell growth and metabolism. This recombinant IGF-1 protein is expressed in CHO cells and corresponds to the active human IGF-1 sequence, ensuring proper folding and biological functionality. With high purity (≥95%) and strong bioactivity (ED50 ≤1.5 ng/mL in MCF-7 cell proliferation assay), this product is ideal for research in oncology, cell biology, and metabolic regulation. The protein is supplied in a tag-free, lyophilized form for convenient storage and reconstitution.
Specifications
| Product Details | |
|---|---|
| Cat. No. | Y03101 |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤10 EU/mg by the LAL method. |
| Bioactivity | Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect ≤1.5 ng/mL. |
| Accession | P05019 |
| Species | Human |
| Expression System | CHO |
| Mol. Weight | 9 kDa |
| Expression Region | MFPAMPLSSLFVN+Gly49-Ala118(E51R) |
| Tag Info | Tag free |
| Formulation | Lyophilized |
| Buffer | Lyophilized from a 0.22 μm filtered solution in Acetic Acid. |
| Size | 10μg/50μg/1mg |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring reliable and reproducible experimental results.
- ✔ High Biological Activity: Demonstrates potent activity with an ED50 ≤1.5 ng/mL in MCF-7 cell proliferation assay, ensuring strong biological performance.
- ✔ Mammalian Expression System: Expressed in CHO cells to ensure correct folding and native-like post-translational modifications.
- ✔ Flexible Application Range: Suitable for a wide range of studies including cancer research, cell proliferation, and signaling pathway analysis.
Applications
Cancer Research
Widely used in tumor biology studies, especially in breast cancer models such as MCF-7 cell proliferation assays.
Metabolism Research
Involved in glucose metabolism, growth regulation, and hormone signaling studies.
Cell Biology
Promotes cell proliferation, survival, and differentiation via IGF-1 receptor-mediated signaling pathways.
Data Figures
Figure 1: Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect ≤1.5 ng/mL. The bioactivity of Y03101 is higher than other competing products.
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
