0 item(s)
View cart
You have no items in your shopping cart.
Request Your Custom Quote Today
Tell us your required quantity and application needs to receive a tailored quotation for this product.
Sku: GMP-TL633-0100
Recombinant Human LIF Protein
Description
The recombinant human leukemia inhibitory factor (LIF) protein is transiently expressed in HEK-293 cells and features a C-terminal His-tag for purification.
As a pleiotropic IL-6 family cytokine, LIF signals through the LIFR-α/gp130 receptor complex, activating the JAK/STAT pathway.
Its core applications in cell and gene therapy include maintaining pluripotency and suppressing spontaneous differentiation of mouse embryonic stem cells (mESCs) for in vitro expansion culture, and promoting the differentiation of neural progenitor cells into cholinergic neurons.
Designed to preserve native bioactivity, this protein is suited for regenerative medicine applications such as stem cell therapy and neural repair.
Specifications
| Product Details | |
|---|---|
| Synonyms | CDF, DIA, HILDA, Leukemia Inhibitory Factor, MLPLI |
| Accession | UniProt P15018 |
| Expressed Region | Ser23–Phe202 |
| Tag | C-terminal 6×His-tag |
| Expression System | HEK293 cells |
| Predicted Molecular Weight | 20.5 kDa |
| Purity | > 95% as determined by SDS-PAGE and HPLC |
| Endotoxin | < 0.01 EU per 1 μg of protein (LAL method) |
| Formulation | Lyophilized from sterile PBS (pH 7.4), typically supplemented with 6% mannitol as a stabilizing protectant |
Features
-
✔
High Purity: Purified using advanced chromatographic techniques, this product achieves a purity level of over 95%, ensuring accurate and reproducible experimental results.
-
✔
High Biological Activity: Validated through multiple activity assays, this LIF protein effectively promotes cell differentiation and survival, making it suitable for a range of research applications.
-
✔
Batch Consistency: Produced under stringent quality control measures to ensure high consistency between batches, reducing variability and enhancing experimental reliability.
- ✔ Animal-Free: Manufactured without the use of animal-derived components, adhering to animal-free standards and broadening its applicability for diverse research needs.
Applications
Stem Cell Research
Study the role of LIF in maintaining the pluripotency of embryonic stem cells and promoting their differentiation into various cell lineages, advancing research in regenerative medicine.
Immunology
Investigate the effects of LIF on immune cell function and differentiation, providing insights into immune responses and potential therapeutic applications.
Oncology
Explore the role of LIF in cancer cell biology, including its impact on tumor growth and metastasis, contributing to the development of novel cancer therapies.
Hematopoiesis
Utilize LIF to study its role in the regulation of hematopoietic stem cells and progenitor cells, offering valuable information for the treatment of hematological disorders.
Stability & Storage
- √ Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
- √ Reconstitution: Dissolve in sterile water for injection, 0.9% NaCl, or PBS (pH7.4), maintaining a final concentration ≥ 100 µg/mL to prevent adsorption.
- √ Handling: Aliquot to avoid repeated freeze-thaw cycles.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Follow recommended storage and handling instructions to maintain product stability.
• Support: Contact technical support if assistance is required.
Document
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
