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$88.00
Sku: Y03021H-1
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by June 06, 2026
Description
Recombinant Human Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine that plays a crucial role in cell differentiation, proliferation, and survival. It belongs to the IL-6 cytokine family and signals through the LIF receptor (LIFR) and gp130 complex, activating downstream pathways such as JAK/STAT3. LIF is widely known for its essential role in maintaining the pluripotency of embryonic stem cells and regulating hematopoiesis and immune responses.
This recombinant protein is expressed in E. coli and corresponds to the mature region (Ser23–Phe202) of human LIF, retaining full biological activity. With high purity (≥95%) and strong bioactivity (ED50 ＜0.5 ng/mL, measured by inhibition of M1 cell proliferation), this product is suitable for stem cell culture, immunology, and cancer research. The protein is supplied in a tag-free, lyophilized form for convenient storage and reconstitution.
Specifications
| Product Details | |
|---|---|
| Cat. No. | Y03001H |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤10 EU/mg by the LAL method. |
| Bioactivity | Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically ＜0.5 ng/mL. |
| Accession | NP_001244064.1 |
| Alternative Names | Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor; D Factor; Melanoma-Derived LPL Inhibitor; MLPLI; Emfilermin; LIF; HILDA |
| Species | Human |
| Expression System | E. coli |
| Mol. Weight | 19.7 kDa |
| Expression Region | Ser23-F202 |
| Tag Info | Tag free |
| Formulation | Lyophilized |
| Buffer | Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, 5% Trehalose and 0.01% Tween 80, pH7.4. |
| Size | 10μg/50μg/1mg |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring reliable and reproducible experimental results.
- ✔ High Biological Activity: Exhibits potent activity with ED50 ＜0.5 ng/mL in M1 cell proliferation inhibition assay, ensuring consistent performance.
- ✔ Optimized Expression System: Expressed in E. coli, enabling efficient production and suitability for routine laboratory applications.
- ✔ Convenient and Reliable: Supplied in a tag-free, lyophilized form for easy reconstitution, stable storage, and compatibility with various experimental workflows.
Applications
Stem Cell Research
Maintains pluripotency and self-renewal of embryonic stem cells via activation of the JAK/STAT3 pathway.
Immunology
Regulates immune responses and cytokine signaling within the IL-6 family network.
Cancer Research
Involved in studies of cell differentiation and proliferation, particularly in leukemia and tumor biology models.
Data Figures
Figure 1: Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically ＜0.5 ng/mL.
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
