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$240.00
Sku: TL-109-0010
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by June 06, 2026
Description
IL-1α is a pleiotropic proinflammatory cytokine and core member of the IL-1 superfamily. Constitutively expressed as a precursor in epithelial cells (particularly dermal keratinocytes), it is proteolytically activated by calpain into a 17-kDa mature form that engages dual receptor mechanisms (IL-1RⅠ signaling/IL-1RⅡ decoy) to trigger NF-κB and MAPK pathways, inducing cascading release of inflammatory mediators including IL-6, COX-2, and nitric oxide (NO).
It exhibits dual immunological functions: acting as an ‘alarmin’ to initiate innate immunity upon tissue injury while maintaining skin barrier integrity against pathogens.
For cell therapy applications, it serves as a critical modulator in ex vivo immune cell maturation:
- In dendritic cell (DC) vaccine production, pulsed low-dose stimulation (pM-fM range) synergizes with TLR ligands to enhance MHC-II/co-stimulatory molecule expression, optimizing tumor antigen presentation;
- For mesenchymal stem cell (MSC) therapeutics, it fine-tunes TRAF6 signaling intensity to induce immunosuppressive HLA-G secretion, augmenting immunomodulatory potency in graft-versus-host disease (GVHD) treatment.
Its activity is reversibly inhibited by IL-1Ra and regulated by nuclear localization sequence (NLS)-mediated translocation, establishing a dynamic equilibrium exploitable for precision interventions in autoimmune disease cell therapies.
Specifications
| Parameter | Specification | Results |
|---|---|---|
| Appearance | Freeze-dried preparation, the form should be white loose body | Compliance |
| Identity | The electrophoretic bands should be consistent with the molecular weight of the product | Compliance |
| Visible Particle | Should be no visible particle | Compliance |
| Moisture | ≤4.0% | 0.7% |
| pH | 6.5~7.5 | 7.2 |
| Purity | >90.0% | Compliance |
| Endotoxin | <0.1EU/μg | <0.1EU/μg |
| Mycoplasma | Should be negative | Negative |
| Sterility | No growth | No growth |
| Biological Activity | Inducing IFN-y secretion in human natural killer lymphoma NK-92 cells through its induction Ability determination, corresponding specific activity≥5x106IU/mg | 5.12x106 IU/mg |
Features
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✔
Defined Recombinant IL-1α Construct
Recombinant human IL-1α (Ser113–Ala271) is expressed in E. coli with a C-terminal 6×His-tag and a predicted molecular weight of 19.2 kDa for consistent handling in research workflows.
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✔
High Purity With Identity Verification
Purity >90% by SDS-PAGE, with electrophoretic bands consistent with the expected molecular weight to support reliable cytokine signaling studies.
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✔
Low Endotoxin and Culture-Ready QC
Endotoxin <0.1 EU/µg (LAL method), mycoplasma negative, and sterility confirmed with no microbial growth for sensitive cell-based assays.
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✔
Bioactivity Validated in NK-92 Cells
Induces IFN-γ secretion in human NK-92 cells with specific activity ≥5 × 106 IU/mg (measured 5.12 × 106 IU/mg) for potency-confirmed experimental use.
Applications
NK-92 IFN-γ Induction Assay
Quantitatively induces IFN-γ secretion in NK-92 cells for IL-1α bioactivity evaluation.
Dendritic Cell Vaccine Research
Supports pulsed low-dose stimulation studies with TLR ligands to enhance MHC-II/co-stimulatory expression.
MSC Immunomodulation Studies
Used to fine-tune TRAF6 signaling intensity and induce HLA-G secretion in MSC-focused GVHD research contexts.
NF-κB/MAPK Signaling Studies
Activates IL-1RⅠ/IL-1RⅡ-driven pathways linked to IL-6, COX-2, and nitric oxide (NO) mediator release.
Stability & Storage
- Lyophilized powder: Stable for 12 months at −80 °C or 6 months at −20 °C when stored in the original sealed container under desiccant.
- Reconstitution: Dissolve contents in 200 µL sterile water for injection, 0.9% NaCl, or PBS (pH 7.4), maintaining a final concentration about 50 µg/mL.
- Handling: Aliquot to avoid repeated freeze–thaw cycles.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Prepare solutions using sterile technique. Avoid microbial contamination to maintain activity.
• Storage: Follow recommended storage conditions. Repeated freeze–thaw cycles may reduce performance.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
