Instant TBS-T Buffer-SuperCulture™

Product Composition

Note: The pH value of 1 × solution is 7.4±0.2@25°C.

Storage and Transportation Conditions

Store at room temperature of 10-30°C, protected from light, with a shelf life of 36 months.

Product Description

Instant TBS-T Buffer is white to off-white instant granules, and each pack of this product can produce 1 L of 1 × TBS-T Buffer, which is easy to operate and use. The main components of TBS-T are sodium chloride, potassium chloride, Tris buffer salt and Tween-20. The concentration of sodium chloride, potassium chloride and Tris buffer salt in 1 × TBS-T Buffer is 137 mM, 2.7 mM and 25 mM respectively, and the content of Tween-20 is 0.05%.
Instant TBS-T Buffer is widely used in biology, which is mainly used in immunohistochemistry, in situ hybridization, ELISA and western blot, and is also commonly used as wash buffer, as well as for antibody dilution and preparation of blocking buffer.

Procedure

1. Place a clean beaker, and add the entire contents of a newly opened pack of Instant TBS-T Buffer.
2. Measure about 500 ml of pure water, slowly add it into the beaker, and gently stir with a stir bar until it is completely dissolved. (This step can also be performed with magnetic stirrer)
3. Transfer the TBS-T solution in Step 2 into a constant volume container, and add water to dilute to 1 L, which is 1 × working solution.

Schematic Diagram of Instant Buffer Preparation