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Description
3,3’,5,5’-Tetramethylbenzidine (TMB) is a sensitive colorimetric substrate widely used in peroxidase and peroxidase-coupled systems such as ELISA. Upon oxidation, TMB produces a blue soluble product, readable spectrophotometrically at 370 nm or 655 nm. The reaction can be stopped with 2 M sulfuric acid, shifting the absorbance to 450 nm and enhancing sensitivity by 2–4 times.
Synonyms:
BM Blue, SureBlue TMB, TMB Substrate, TMB Blotting Plus
Specifications
| Test Item | Specification | Result |
|---|---|---|
| Appearance | White or light yellow crystal | Conforms |
| Assay (HPLC) | ≥ 99.0% | Conforms |
| Melting Point | 168.0 – 171.0 °C | Conforms |
| TLC | One Spot | Conforms |
| Heavy Metals | ≤ 5 ppm | Conforms |
| Loss on Drying | ≤ 0.5% | Conforms |
Number of Containers: 1
Storage: 2 – 8 °C (Refrigerator)
Shipping Conditions: ICE
Sterile: No
DG Classification: Not Dangerous Goods
UNSPSC Code:12161500
UNSPSC Category: Indicators
CAS: [54827-17-7]
Grade: High Purity
Documents
Disclaimer: For laboratory research use only.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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