MaxSortin® CD138 Isolation Kit

Composition

Specification

Intended Use

MaxSortin® CD138 Separation Magnetic Beads can be used to sort CD138+ cells. By coupling anti-human CD138 monoclonal antibodies onto the magnetic beads, incubating them with cells, and then performing magnetic separation, the sorting of CD138+ cells can be achieved.

Experimental Procedures

1. Resuspend the mixed cell sample of PBMCs containing about 10% U266 in PBS buffer containing 1% human serum albumin (HSA). Take a sample for counting and transfer 1×10⁷ cells into a 1.5 mL Ep tube. Then centrifuge at 1500 rpm for 5 minutes.

2. Discard the supernatant, take 90 μL of MaxSortin® Cell Sorting Buffer solution to resuspend the cells, add 10 μL of MaxSortin® CD138 Separation Magnetic Beads, mix well and then place them in a refrigerator at 2 - 8 °C for incubation for 15 minutes.

3. Take the MaxSortin® L-Separation Column and place it on the magnetic-activated cell sorting (MACS) sorter, and rinse it twice with 1 mL of MaxSortin® Cell Sorting Buffer.

4. Take the sample that has completed incubation out of the refrigerator at 2 - 8 °C, add 1 mL of MaxSortin® Cell Sorting Buffer, centrifuge at 1500 rpm for 5 minutes, and discard the supernatant.

5. Add 1 mL of MaxSortin® Cell Sorting Buffer to resuspend the cells, add the sample into the MaxSortin® L-Separation Column. After it flows out naturally, add MaxSortin® Cell Sorting Buffer in two portions, 3 mL each time, to wash the cells adhering to the column.

6. After all the MaxSortin® Cell Sorting Buffer has flowed out, remove the MaxSortin® L-Separation Column from the MACS sorter and place it in a 15 mL centrifuge tube (mark it properly). Add 3 mL of MaxSortin® Cell Sorting Buffer into the MaxSortin® L-Separation Column and use the piston to push the liquid out directly. The collected cells are the CD138+ cells.

7. Count the cells and conduct flow cytometry detection.

Precautions

• When incubating the magnetic beads with cells, it is necessary to mix them thoroughly to improve the sorting efficiency.

• When the proportion of CD138+ cells is below 3%, it is recommended to add 5 μL of magnetic beads. When the proportion of CD138+ cells is above 3%, it is recommended to add 10 μL of magnetic beads.

• This product cannot be used for clinical treatment.