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Introduction
| Components | Shipping Condition | Unit Size | Product Line | Detection Method |
| Plasmid linear DNA Control | -20℃ | 100 Reactions | SHENTEK™ | Probe-based qPCR |
| qPCR Master MIX | ||||
| DNA Dilution Buffer (DDB) | ||||
| Plasmid Primer&Probe MIX (Incl IPC) | -20℃, protect from light |
Overview
SHENTEK™ Plasmid DNA Quantitation Kit is used to quantitate plasmid DNA residues in gene therapy products, such as the plasmid DNA of lentiviral vector preparation in CAR-T cell therapy. This kit uses duplex real-time PCR technology to detect trace amounts of residual plasmid DNA by means of consensual plasmid sequences, such as replicon of ColE1/pMB1/pBR322/pUC. The target gene (FAM) performs rapid, specific, and reliable quantitative determination of the 102 copies/μL level of residual Plasmid DNA. IPC—Internal Positive Control (VIC) is included in the Plasmid Primer&Probe MIX to evaluate the performance of each PCR reaction. Customers can send DNA sequence of empty plasmid backbones to our technicians for confirmation in advance. For extraction information, please refer to the SHENTEK™ Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).
Specification
| Range | 4.97-4.97x106 copies/μL, R2=0.99968, AE=94.3% |
| Accuracy | Recovery = 88.4%-115.2% , CV<30% |
| LOQ | 4.97 copies/μL |
| LOD | 1.66 copies/μL |
| Precision | 3.3%-17.8% (<30%) |
| Robustness | Freeze-thaw stability over 5 cycles |
| Instrument suitability not limited to Thermo, Biorad, Roche, HZSKBio qPCR equipments |
Related Products
SHENTEK™ Residual Host Cell DNA Sample Preparation Kit
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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