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Description
Bio Basic’s Lysozyme for Molecular Biology is a high-purity, triple-crystallized enzyme designed for efficient cell wall lysis, particularly in Gram-positive bacteria. With a protein content of ≥90% and specific activity ≥20,000 units per milligram, this lyophilized preparation is ideal for DNA extraction, structural biology, and proteomics workflows. The enzyme hydrolyzes the β(1→4) glycosidic bonds between N-acetylmuramic acid and N-acetyl-D-glucosamine in bacterial cell walls, and is also effective on chitosan oligomers.
Specifications
| Grade | Biotech |
| CAS | 12650-88-3 |
| Appearance | White to off-white granulate |
| Solubility (2%-aqua at 640nm) | ≥95% |
| Purity on dry basis | ≥95% |
| Activity in FIP Units/mg protein | ≥39000 |
| pH (2%-aqua) | 3.0-3.6 |
| Water content | ≤6% |
| Residue on ignition | ≤1.5% |
| Storage | -20℃ |
Features
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High-purity, triple-crystallized lysozyme supplied as a lyophilized preparation
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Hydrolyzes β(1→4) glycosidic bonds between N-acetylmuramic acid and N-acetyl-D-glucosamine in bacterial cell walls
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Particularly effective for the lysis of Gram-positive bacteria
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Also effective on chitosan oligomers
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Suitable for DNA extraction, structural biology, and proteomics workflows
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Compatible with boiling lysis protocols
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Lyophilized preparation is stable for ≥4 years at -20°C
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Reconstituted preparation at pH 4–5 is stable for weeks at 2–8°C
Applications
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Bacterial cell lysis for plasmid DNA extraction
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Structural and functional studies of proteins
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Standard protein for mass spectrometry calibration
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Compatible with boiling lysis protocols
Documents
FAQ
What is Bio Basic’s Lysozyme used for?
It is used for bacterial cell wall lysis, plasmid DNA extraction, protein studies, and mass spectrometry calibration.
How does the enzyme break down bacterial cell walls?
It hydrolyzes the β(1→4) glycosidic bonds between N-acetylmuramic acid and N-acetyl-D-glucosamine.
How should the lysozyme be dissolved?
Dissolve at 10 mg/mL in distilled water. Reconstitution in 10 mM Tris-HCl, pH 8.0, is recommended.
How should the product be stored?
The lyophilized product is stable for ≥4 years at -20°C. When reconstituted at pH 4–5, it is stable for weeks at 2–8°C.
Is this product intended for clinical use?
No. It is for laboratory research use only.
Disclaimer: For laboratory research use only.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.

