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For laboratory research use only.
$96.00
Sku: DD0099-250mg
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by June 22, 2026
Description
DNase I (DD0099) is a chromatographically purified endonuclease derived from bovine pancreas that efficiently digests double-stranded DNA into oligonucleotides containing 3'-OH termini. In the presence of Mg²⁺ ions, DNase I introduces single-strand nicks, while Mn²⁺ enables cleavage of both DNA strands.
This high-activity DNase I (>500 Kunitz U/mg) is widely used for:
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Removal of genomic DNA contamination from RNA samples
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RNA preparation prior to RT-PCR and qPCR
-
Single-cell suspension preparation from tissues
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Cell isolation workflows
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Nick translation experiments
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Random DNA fragmentation
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Molecular cloning applications
The enzyme is supplied as a lyophilized powder and provides reliable DNA digestion performance in molecular biology, cell biology, immunology, and cancer research laboratories.
Specification
| Source | Bovine Pancreas |
| CAS | 9003-98-9 |
| Appearance | White to off-white crystalline powder |
| Activity | >500Kunitz U/mg |
| Loss on Drying | ≤5.0% |
| Residue on Ignition | ≤1.0% |
| Storage | -20℃. Keep dry. Warm to room temperature before opening. |
Important Usage Considerations
For optimal RNA integrity, follow the recommended digestion conditions provided in the product protocol.
Applications
Biofargo DNase I is commonly used in:
-
RNA Purification
Remove residual genomic DNA contamination before reverse transcription and downstream RT-PCR analysis. -
RT-PCR and qPCR Sample Preparation
Improve amplification accuracy by eliminating contaminating DNA from RNA samples. -
Single Cell Suspension Preparation
Reduce extracellular DNA released from dead cells during tissue dissociation and cell isolation procedures. -
Immunology Research
Frequently used in PBMC isolation, splenocyte preparation, lymphocyte isolation, and immune cell culture workflows. -
Cancer Research
Suitable for tumor tissue dissociation and preparation of single-cell suspensions for gene expression studies. -
Molecular Cloning
Generate random DNA fragments and perform nick translation experiments.
QC Results
Lane 1: Marker
Lane 2: Negative control (no DNase I) Lane 3: DNA + 1 unit DNase I
Lane 4: DNA + 2 units DNase I
Lane 5: DNA + 3 units DNase I
Documents
FAQ
Does Biofargo DNase I contain an RNase inhibitor?
No, this DNase I does not include an RNase inhibitor. For RNA-sensitive applications, please use RNase-free reagents and handling procedures.
What is DNase I used for?
DNase I is used to digest DNA during RNA purification, tissue dissociation, cell isolation, molecular cloning, and RT-PCR sample preparation.
Can DNase I remove genomic DNA before RT-PCR?
Yes. DNase I efficiently removes contaminating genomic DNA from RNA samples before reverse transcription and PCR analysis.
Is DNase I suitable for single-cell preparation?
Yes. DNase I is commonly used during tissue dissociation to reduce DNA-mediated cell clumping and improve single-cell suspension quality.
What is the activity of Bio Basic DNase I?
Biofargo DNase I provides activity greater than 500 Kunitz Units per mg.
What is the source of this DNase I?
This enzyme is derived from bovine pancreas.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
