Inhibitor of Residual RT Activity of DNA Polymerase

Description

This product is intended for inhibiting the residual reverse transcriptase activity of DNA polymerases in PERT assays.

PERT (Polymerase Extension Reverse Transcription) is an extremely sensitive method for detecting reverse transcriptase activity. However, samples—particularly cell lysates and cell culture supernatants—often contain DNA polymerases. Notably, many prokaryotic DNA polymerases (e.g., Taq DNA polymerase) as well as eukaryotic DNA polymerases exhibit residual reverse transcriptase activity. Such activity significantly increases background signals, leading to false-positive results even in negative controls and severely interfering with the assay. It has the following features:

• Ready-to-use: Add to PERT reaction system at specified ratio.

• Specifically inhibits DNA polymerase's reverse transcriptase activity; no effect on most reverse transcriptases.

• Compatible with all DNA polymerase types.

• Inhibitory effect varies by DNA polymerase, typically ~10-fold (Ct value increases by ~3).

• 1 mL sufficient for 500 × 20 μL PERT reactions.

• Research use only, not for diagnostic use.

Kit Components

Storage and Handling

Transport at low temperature, store at -20℃, the storage period is 12 months.

Materials Required but not Supplied in the Kit

Sample, Ultra-pure water, Reverse Transcriptase Assay (PERT)

Procedures

Prior to reverse transcription, add 1/10 volume of this product to the PERT reaction system (e.g., 2 μL for a 20 μL system). Follow standard protocols for the rest of the procedure. It is recommended to include a control without this product to verify efficacy. The dosage can be adjusted above or below the 1/10 volume baseline for optimization.

Related Products

Reverse Transcriptase Assay (PERT)

Limited Product Warranty

• The information in this guide is subject to change without notice.

• This product is intended for research purposes only. This product is not intended to be used for therapeutic and diagnostic purposes in humans or animals.

• DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, BIOFARGO WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.

Documents

Manual