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Description

The Aptamer Screening Experimental Kit is an integrated laboratory reagent and consumable system designed to support nucleic acid-based affinity screening experiments. The kit consolidates all essential reagents, buffers, amplification mixes, electrophoresis materials, and supporting tools required to perform iterative nucleic acid binding, amplification, separation, and recovery workflows in a single package.

All components are provided in ready-to-use formats and are optimized for compatibility with magnetic bead handling, PCR and qPCR amplification, denaturing PAGE separation, and nucleic acid purification procedures. This kit is intended to simplify experimental setup, reduce sourcing complexity, and improve workflow consistency for laboratories conducting nucleic acid screening and enrichment experiments.

Features

  • Comprehensive reagent coverage: All key reagents, buffers, and consumables required for nucleic acid screening workflows are included.
  • Workflow consistency: Reduces variability caused by sourcing reagents from multiple suppliers.
  • Ready-to-use system: Components are supplied in formats suitable for direct laboratory use.
  • Compatibility with standard equipment: Designed to work with commonly available magnetic racks, thermocyclers, electrophoresis systems, and imaging devices.
  • Modular experimental support: Individual components can be used independently or as part of an integrated workflow.

Applications

Suitable for research laboratories performing:

  • Nucleic acid binding and enrichment experiments
  • Affinity screening and molecular interaction studies
  • Optimization of nucleic acid amplification workflows
  • PAGE-based nucleic acid separation and analysis
  • Experimental method development and validation
  • Training, teaching, and protocol standardization

Applications may vary depending on user-defined experimental design and target systems.

Components

Component Cat. No.
Carboxyl Magnetic Beads SEP-201903-1
ssDNA Library (11 bp Fixed Region + 80 nt Random Region) SEP-201903-2
Sodium Acetate Buffer (pH 4.0) SEP-201903-3-1
Sodium Acetate Buffer (pH 4.5) SEP-201903-3-2
Sodium Acetate Buffer (pH 5.0) SEP-201903-3-3
EDC SEP-201903-4
NHS SEP-201903-5
Ethanolamine, 1 M (pH 8.5) SEP-201903-6
BSA (1 mg/mL) SEP-201903-7
PCR Mix ESE-201801-4
qPCR Mix ESE-201801-5
0.5 mL Centrifuge Tubes ESE-201801-6
Emulsion PCR Droplet Oil EPO-100
Denaturing PAGE Running Buffer DDP-100-1
10% APS (Ammonium Persulfate) DDP-100-2
TEMED DDP-100-3
2× TBE Urea Loading Buffer TLB-5
Fluorescent ssDNA Ladder SDL-100
Micro Nucleic Acid Dialysis Membrane MDM-30-1
Micro Nucleic Acid Dialysis Device MDM-40-2
Preparative Electrophoresis Comb
Single-edge Razor Blade
Cotton Thread
UV Gel Cutting Flashlight
Flat-tip Carbon Fiber Tweezers
Strong Magnet
Small Scissors
Water Bath Float
1.5 mL Tube Safety Clip

Experimental Workflow Compatibility

Supports the following experimental operations:

Magnetic bead immobilization
Chemical coupling reactions
PCR and qPCR amplification
Emulsion PCR workflows
Denaturing PAGE
Nucleic acid size analysis
Gel extraction & recovery
Dialysis purification

Notes:

  • This product is supplied for Research Use Only (RUO).
  • Experimental outcomes depend on user-defined protocols and conditions.
  • This kit does not include targets or experimental design services.
  • Follow standard laboratory safety and handling practices.

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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