Blood SuperDirect™ PCR Kit (Heparin)

Description

This product uses a unique PCR buffer system, which allows direct PCR with whole blood samples as a template without DNA extraction or sample processing. EDTA, Heparin anticoagulated whole blood and blood collection cards (such as Whatman 903® and FTA® blood collection cards) can be directly used for PCR amplification and identification, which greatly shortens the detection time. The 2× SuperEasyTM Mix provided by this kit has strong inhibitor tolerance. The maximum amount of human blood used as a PCR template can be up to 45% of the PCR system and 20% of mouse blood, which can sensitively amplify blood samples. Genomic and foreign target DNA fragments.

2× SuperEasyTM Mix-Heparin contains our company’s unique Foregene D-Taq DNA Polymerase, dNTPs, MgCl2, Taq Reaction Buffer, PCR reaction enhncer, optimizer and stabilizer.

Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

According to the different anticoagulants used, this product is divided into two categories: EDTA anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-EDTA) and Heparin anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-Heparin); You can also choose the corresponding kit according to the needs of the experiment, see the selection of kit type for details.

Feature

• No need for time-consuming and expensive DNA purification, no pretreatment, and direct use of whole blood as a PCR template.

• The system has strong amplification ability and can sensitively detect the genome and foreign target DNA fragments in the blood.

• 2× SuperEasyTM Mix-Heparin High blood tolerance: up to 45% in human blood and 20% in mouse blood.

• The sample is fully enclosed operation, so there is no need to worry about sample contamination and false positive PCR results.

Work Flow

Storage

This kit should be stored at -20℃.

• 2× SuperEasyTM Mix-Heparin,If used frequently, it can be stored at 4°C for short-term storage (use up within 10 days).

• 6× DNA Loading Buffer,It can be stored at 4°C or -20°C for a long time.