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99+% (TMB) CAS 54827-17-7
$26.21
Sku: TB0954-1g
Categories:
Activity Collection, Chemicals, Chemicals (S-Z), Misc. Biochemicals
Description
3,3’,5,5’-Tetramethylbenzidine (TMB) is a sensitive colorimetric substrate widely used in peroxidase and peroxidase-coupled systems such as ELISA. Upon oxidation, TMB produces a blue soluble product, readable spectrophotometrically at 370 nm or 655 nm. The reaction can be stopped with 2 M sulfuric acid, shifting the absorbance to 450 nm and enhancing sensitivity by 2–4 times.
Synonyms:
BM Blue, SureBlue TMB, TMB Substrate, TMB Blotting Plus
Specifications
| Grade | High Purity |
| Formula | C16H20N2 |
| C16H20N2 | 240.4 |
| CAS | 54827-17-7 |
| Appearance | White or light yellow crystal |
| Assay (HPLC) | ≥99.0% |
| Melting point | 168.0 – 171.0℃ |
| TLC | One spot |
| Heavy metals | ≤5ppm |
| Loss on drying | ≤0.5% |
| Storage | 4℃ |
Documents
Disclaimer: For laboratory research use only.
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