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$57.31
Sku: PB0451-50mg
Categories:
Activity Collection
, Chemicals
, Chemicals (M-R)
, Protease/Phosphatase Inhibitors
Specifications
| Grade | Biotech |
| CAS | 39450-01-6 |
| Appearance | White lyophilized powder |
| Activity(hemoglobin:ph7.5;37℃) | ≥30.0 mAnsonU/mg protein |
| DNase(Nlcking activity;Pbr 322; 37℃) | None detectable |
| RNase activity | None detectable |
| Exonucleases | None detectable |
| Storage | -20ºC. Protect from moisture. Warm to room temperature before opening |
Application
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Biochem/physiol Actions
Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
Documents
Disclaimer: For laboratory research use only.
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