Nitrotetrazolium Blue Chloride (NBT) 90% High Purity Grade

Description

Nitrotetrazolium Blue Chloride (NBT) is a high-purity yellow crystalline dye widely used in biochemical and cell-based assays. NBT functions as an electron acceptor, forming a dark blue insoluble formazan upon reduction, making it a powerful tool for detecting enzymatic activity and cellular metabolic processes. Its reliable performance allows researchers to visualize and quantify enzyme reactions, particularly in histochemistry, cytochemistry, and microbiological studies.

Supplied as a yellow crystalline powder with high solubility in water (10 mg/mL), NBT enables consistent and reproducible results in both qualitative and quantitative assays. Its high purity (≥90% by HPLC) ensures minimal interference in experimental procedures, making it an essential reagent for laboratory research in enzymology, cell biology, and related fields.

Specifications

Features

• High Purity: ≥90% (HPLC), ensuring accurate and reproducible assay results.

• Reliable Enzyme Detection: Forms dark blue formazan upon reduction, suitable for measuring enzymatic activity.

• Water Soluble: Readily dissolves in water to form a clear yellow solution for experimental use.

• Versatile Applications: Suitable for histochemical, cytochemical, microbiological, and biochemical studies.

• Stable Yellow Crystalline Powder: Melting point ≥184°C, providing convenient storage and handling in the lab.

Applications

• Enzyme Activity Assays: Visualize and quantify enzyme reactions in vitro.

• Histochemical and Cytochemical Staining: Detect metabolic activity in tissue sections and cultured cells.

• Microbiological Analysis: Identify microbial enzymatic activity and metabolic processes.

• Cell Viability Studies: Assess cellular respiration and redox activity in research assays.

• Educational and Analytical Use: Demonstrate enzyme-mediated color changes in teaching laboratories.

Documents

COA

SDS

Disclaimer: For laboratory research use only.