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$12.00
Sku: S0593-5g
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by August 18, 2026
Description
Unwanted autofluorescence can interfere with fluorescent labels in fixed-tissue imaging, while lipid and lipoprotein workflows require a dye with documented preparation and staining conditions. Sudan Black B, Product Code S0593, is an Indicator Grade laboratory dye supplied as a very dark brown to black crystalline powder for laboratory research use.
The product information reports the use of Sudan Black B for reducing lipofuscin-like autofluorescence in mammalian neural tissue and reducing autofluorescence in archival formaldehyde-fixed, paraffin-embedded myocardium tissue. Other reported applications include detecting native and oxidized low-density lipoproteins after capillary isotachophoresis, staining fat in animal tissues and bacteria, staining the lipid portion of lipoproteins after polyacrylamide gel electrophoresis, and staining lipofuscin.
Sudan Black B is soluble in ethanol at 10 mg/mL and may require heat for complete solubilization. It is also soluble in acetone, benzene, toluene, hydrocarbon solvents, fats, oils, and paraffins, and is slightly soluble in water at 0.1 mg/mL.
Specification
| Product Name | Sudan Black B |
| Product Code | S0593 |
| Grade | Indicator |
| CAS No. | 4197-25-5 |
| EC No. | 224-087-1 |
| Synonyms | Solvent Black B1; Fat Black HB; Solvent Black 3 |
| Molecular Formula | C29H24N6 |
| Molecular Weight | 456.55 |
| Appearance | Very dark brown to black crystalline powder |
| Identification | Conforms to structure and standard |
| Melting Point | 118–126 °C |
| Dye Content | 100 ± 5% |
| Loss on Drying | <5.0% |
| Absorption Maximum | λmax = 596–605 nm |
| COA λmax Specification | 598 (412) nm in ethanol |
| Extinction Coefficient | E1% = 575–630 at 596–605 nm in ethanol |
| Solubility | Ethanol: 10 mg/mL; heat may be required for complete solubilization Water: slightly soluble at 0.1 mg/mL Also soluble in acetone, benzene, toluene, hydrocarbon solvents, fats, oils, and paraffins |
| Storage | Room temperature; tightly closed, dry, and protected from moisture |
| Recommended Use | For laboratory research use only |
Features
- Indicator Grade: Supplied as Sudan Black B Indicator Grade under Product Code S0593.
- Defined Physical Specifications: Very dark brown to black crystalline powder with a specified melting point of 118–126 °C.
- Documented Spectral Properties: Product information specifies a λmax range of 596–605 nm and an extinction coefficient of E1% = 575–630 in ethanol.
- Documented Preparation Information: Soluble in ethanol at 10 mg/mL, although heat may be required for complete solubilization.
- Reported Research Applications: Product information describes applications involving fat, lipoproteins, lipofuscin, LDLs, and autofluorescence reduction.
- Room-Temperature Storage: Store tightly closed in a dry area and protect from moisture.
- Transport Classification: The SDS lists the product as not regulated as dangerous goods under TDG, IMDG, or IATA requirements.
Applications
- Lipid and Fat Staining: Reported for staining fat in animal tissues.
- Bacterial Fat Staining: Reported for staining fat in bacteria using Burdon’s method.
- Lipoprotein Staining after PAGE: Used to stain the lipid portion of lipoproteins following polyacrylamide gel electrophoresis.
- Native and Oxidized LDL Detection: Used to detect native and oxidized low-density lipoproteins following capillary isotachophoresis.
- Lipofuscin Staining: Reported for staining lipofuscin.
- Neural Tissue Autofluorescence Reduction: Reported to eliminate lipofuscin-like autofluorescence in mammalian neural tissue without adversely affecting other fluorescent labels.
- Fixed-Tissue Autofluorescence Reduction: Reported to reduce autofluorescence in archival formaldehyde-fixed, paraffin-embedded myocardium tissue.
These applications are reported in the supplied product information. Users should determine and validate suitability for their samples, procedures, and laboratory conditions.
Preparation Instructions and PAGE Procedure
Sudan Black B is soluble in ethanol at 10 mg/mL and yields a dark blue solution. Heat may be required for complete solubilization. It is also soluble in acetone, benzene, toluene, hydrocarbon solvents, fats, oils, and paraffins, and is slightly soluble in water at 0.1 mg/mL.
For staining the lipid portion of lipoproteins after polyacrylamide gel electrophoresis:
- Prepare a staining solution containing 500 mg Sudan Black B in 20 mL acetone.
- Add the mixture to 15 mL acetic acid, and then add it to 85 mL water.
- Stir for 30 minutes and centrifuge to remove the precipitate.
- Stain the gel overnight in the prepared solution.
- Destain the gel using three changes of a solution containing 150 mL acetic acid, 200 mL acetone, and 650 mL water.
Review the SDS before preparation or handling. Avoid inhalation of dust and substance contact. Wear protective gloves, protective clothing, and eye/face protection. Respiratory protection is required when dust is generated.
Warning: Suspected of causing genetic defects (H341).
Documents
FAQ
What grade is Sudan Black B S0593?
Sudan Black B S0593 is supplied as Indicator Grade. The supplied documents do not identify it as BSC certified, high-purity biological stain, histology grade, or microscopy grade.
What is the CAS number of Sudan Black B?
The CAS number is 4197-25-5. The molecular formula is C29H24N6, and the molecular weight is 456.55.
What are the synonyms for Sudan Black B?
The product information lists Solvent Black B1, Fat Black HB, and Solvent Black 3 as synonyms.
Is Sudan Black B soluble in ethanol?
Yes. Sudan Black B is soluble in ethanol at 10 mg/mL and yields a dark blue solution. Heat may be required for complete solubilization.
Is Sudan Black B soluble in water?
Sudan Black B is slightly soluble in water at 0.1 mg/mL. It is also soluble in acetone, benzene, toluene, hydrocarbon solvents, fats, oils, and paraffins.
What Sudan Black B applications are reported in the product information?
Reported applications include staining fat in animal tissues and bacteria, staining the lipid portion of lipoproteins after PAGE, detecting native and oxidized LDLs after capillary isotachophoresis, staining lipofuscin, and reducing autofluorescence in specified fixed-tissue applications.
Can Sudan Black B reduce lipofuscin-like autofluorescence?
The product information reports that Sudan Black B was found to eliminate lipofuscin-like autofluorescence in mammalian neural tissue without adversely affecting other fluorescent labels. It was also reported to reduce autofluorescence in archival formaldehyde-fixed, paraffin-embedded myocardium tissue. Suitability should be validated for each sample and imaging workflow.
How should Sudan Black B be stored?
Store at room temperature in a tightly closed container. Keep the product dry, protect it from moisture, and store it in a locked or restricted-access area.
What safety precautions apply to Sudan Black B?
The SDS classifies the product as Germ Cell Mutagenicity Category 2 and lists H341: Suspected of causing genetic defects. Avoid inhalation of dust and substance contact, and wear suitable protective gloves, protective clothing, and eye/face protection.
Is Sudan Black B intended for diagnostic use?
No. It is for laboratory research use only and is not intended for human or animal diagnostic or therapeutic use.
Where can I find the INFO, COA, and SDS for Sudan Black B?
The Product Information, COA, and Safety Data Sheet are provided in the Documents section.
For laboratory research use only. Not intended for human or animal diagnostic or therapeutic use.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
