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Description
Laboratory protocols that specify a defined phosphate reagent require clear confirmation of the compound identity, grade, composition, storage conditions, and quality-control method before use. pNPP-Na is supplied as reagent-grade p-nitrophenyl phosphate disodium salt hexahydrate for laboratory research use.
This material is provided as a white-to-yellow crystalline powder with the molecular formula O2NC6H4OP(O)(ONa)2 · 6H2O, a molecular weight of 371.14, and CAS number 333338-18-4. The product specification lists a quantitative 1H-NMR result of ≥95%.
Compound identity is evaluated by 1H-NMR, 13C-NMR, and 31P-NMR spectra corresponding to the assigned structure. These documented specifications help researchers confirm that the supplied pNPP-Na matches the material required by their validated laboratory procedure.
Store the tightly closed container at −20°C in a dry, well-ventilated location and protect the material from light. This product is intended for laboratory research use only and is not intended for human or animal drug use.
Product Identification
| Product Name | pNPP-Na |
| Chemical Name | p-Nitrophenyl Phosphate Disodium Salt Hexahydrate |
| Alternative Formatting | p-Nitrophenyl Phosphate Disodium Hexahydrate; pNPP Disodium Hexahydrate |
| Product Code | NB0365 (P0364) |
| Grade | Reagent Grade |
| CAS Number | 333338-18-4 |
| EC Number | 224-246-5 |
Specification
| Molecular Formula | O2NC6H4OP(O)(ONa)2 · 6H2O |
| Molecular Weight | 371.14 |
| Color | White to yellow |
| Form | Crystalline powder |
| Quantitative 1H-NMR | ≥95% |
| 1H-NMR Spectrum | Corresponds to assigned structure |
| 13C-NMR Spectrum | Corresponds to assigned structure |
| 31P-NMR Spectrum | Corresponds to assigned structure |
| Recommended Storage | −20°C |
| Light Sensitivity | Light sensitive; protect from light |
| Recommended Use | For laboratory research use only |
Features
- Defined chemical identity: Supplied as p-nitrophenyl phosphate disodium salt hexahydrate.
- Reagent-grade material: Identified as reagent grade in the product specification.
- Quantitative NMR specification: ≥95% by quantitative 1H-NMR.
- Multi-nucleus structural confirmation: Assigned structure evaluated by 1H, 13C, and 31P NMR.
- Documented physical form: White-to-yellow crystalline powder.
- Defined storage requirement: Store at −20°C in a tightly closed container and protect from light.
Applications
pNPP-Na is intended for laboratory research workflows in which p-nitrophenyl phosphate disodium salt hexahydrate is specifically required by an established or user-validated procedure.
- Laboratory research protocols specifying pNPP-Na as a chemical reagent.
- Preparation of research reagents where the protocol requires p-nitrophenyl phosphate disodium salt hexahydrate.
- Method development or method verification performed under laboratory-defined conditions.
- Research workflows requiring a reagent-grade crystalline form with documented NMR specifications.
Important: The supplied COA and SDS do not specify assay compatibility, working concentration, reaction conditions, detection wavelength, enzyme specificity, or validated performance in a particular method. Researchers should determine suitability using their own protocol and validation criteria.
Storage and Handling
- Store at the recommended temperature of −20°C.
- Keep the container tightly closed in a dry, well-ventilated place.
- Protect the product from light because exposure to light may affect product quality.
- Avoid dust formation and use appropriate exhaust ventilation where dust may be generated.
- Use suitable laboratory eye protection and handle the material with gloves.
- Avoid contact with strong oxidizing agents, strong acids, and strong bases.
- Handle in accordance with good laboratory hygiene and safety practices.
Safety and Transport Information
| Hazard Classification | Not classified as a hazardous substance or mixture in the supplied SDS |
| DOT | Not dangerous goods |
| IMDG | Not dangerous goods |
| IATA | Not dangerous goods |
| Environmental Precaution | Do not allow the product to enter drains |
| Use Limitation | For research use only; not intended for human or animal diagnostic or therapeutic use |
Documents
FAQ
What is pNPP-Na?
pNPP-Na is p-nitrophenyl phosphate disodium salt hexahydrate. It is supplied as a reagent-grade crystalline powder for laboratory research use.
What are the molecular formula and molecular weight of pNPP-Na?
The molecular formula is O2NC6H4OP(O)(ONa)2 · 6H2O, and the molecular weight is 371.14.
What is the CAS number for p-nitrophenyl phosphate disodium salt hexahydrate?
The CAS number listed for this product is 333338-18-4.
What grade and physical form are supplied?
The product is supplied as reagent-grade material. Its specified appearance is a white-to-yellow crystalline powder.
What does the ≥95% specification mean?
The Certificate of Analysis lists a quantitative 1H-NMR specification of ≥95%. The document does not identify this value as an HPLC, enzymatic-activity, or assay-performance result.
How is the identity of pNPP-Na confirmed?
The product specification states that the 1H-NMR, 13C-NMR, and 31P-NMR spectra correspond to the assigned structure.
How should pNPP-Na be stored?
Store pNPP-Na at −20°C. Keep the container tightly closed in a dry, well-ventilated place and protect the product from light.
Is pNPP-Na light sensitive?
Yes. The Safety Data Sheet identifies the material as light sensitive and states that exposure to light may affect product quality.
Is this product classified as hazardous?
The supplied Safety Data Sheet does not classify the product as a hazardous substance or mixture. Standard laboratory protective practices should still be followed.
Is pNPP-Na regulated as dangerous goods for transport?
The SDS lists the product as not dangerous goods under DOT, IMDG, and IATA transport classifications.
Can this pNPP-Na be described as an ELISA or alkaline phosphatase substrate?
The supplied COA and SDS identify the chemical and its quality and safety specifications, but they do not provide a validated ELISA claim, alkaline phosphatase assay claim, enzyme-activity specification, working concentration, or detection wavelength. Suitability for a specific assay must therefore be established by the user's validated protocol.
Is solubility information available for this product?
No solubility value or recommended reconstitution procedure is provided in the supplied COA or SDS. Researchers should follow an established method and confirm suitability under their own experimental conditions.
Is pNPP-Na intended for clinical, diagnostic, or therapeutic use?
No. This product is for laboratory research use only and is not intended for human or animal diagnostic or therapeutic use.
For laboratory research use only. Not intended for human or animal diagnostic or therapeutic use.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.

