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Indicator-grade powder for preparing calcium staining reagents in laboratory research.
$26.00
Sku: AD0144-25g
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by August 07, 2026
Description
Alizarin Red S is an anthraquinone derivative supplied as an indicator-grade powder for laboratory research use. According to the supplied product information, it may be used to identify calcium in neutral buffered formalin-fixed or alcoholic formalin-fixed, paraffin-embedded tissue sections.
Calcium forms an Alizarin Red S-calcium complex through a chelation process. When the supplied histological staining procedure is followed, calcium deposits, except oxalate, appear orange-red, and the resulting precipitate is birefringent.
The staining reaction is not strictly specific for calcium. Magnesium, manganese, barium, strontium, and iron may interfere, although the product information states that these elements do not usually occur in sufficient concentrations to interfere with staining.
This product is supplied as a powder and is not a ready-to-use staining solution. Users should prepare the staining solution and follow an appropriately validated procedure for their tissue type and experimental conditions.
Specifications
| Product Name | Alizarin Red S |
| SKU | AD0144 |
| Package Size | 25 g |
| Form | Powder |
| Grade | Indicator |
| CAS No. | 130-22-3 |
| EC No. | 204-981-8 |
| Molecular Formula | C14H7O7SNa |
| Molecular Weight | 342.26 |
| Appearance | Yellow-green to orange-brown powder |
| Infrared Spectrum | Conforms to Structure |
| Absorbance Ratio P-15/P+15 | 0.97-1.07 |
| Lambda Max | 554-558 nm |
| Lambda Max | 590-599 nm |
| Purity (HPLC) | Reported on the applicable Certificate of Analysis |
| Storage | Room temperature; keep tightly closed in a dry, well-ventilated place |
| Recommended Use | For laboratory research use only |
Features
- Indicator-Grade Powder: Supplied as a powder for laboratory preparation of Alizarin Red S staining solution.
- Documented Histological Application: The supplied product information describes calcium staining in formalin-fixed, paraffin-embedded tissue sections.
- Orange-Red Staining Result: Calcium deposits, except oxalate, appear orange-red when the supplied histological procedure is followed.
- Birefringent End Product: The precipitate produced by the staining reaction is described as birefringent.
- Defined Preparation Conditions: The supplied procedure specifies a 2% w/v staining solution adjusted to pH 4.1-4.3.
- Room-Temperature Storage: Store tightly closed in a dry, well-ventilated place at room temperature.
Documented Application
According to the supplied product information, Alizarin Red S may be used to identify calcium in fixed, paraffin-embedded tissue sections.
- Sample Type: Paraffin-embedded tissue sections
- Fixation: Neutral buffered formalin or alcoholic formalin
- Positive Control: Mouse embryo or a known calcium-containing tissue section
- Expected Result: Calcium deposits, except oxalate, appear orange-red
- Microscopic Observation: The resulting precipitate is birefringent
Application note: For applications outside the supplied histological procedure, users should determine product suitability using an appropriately validated protocol.
Histological Staining Solution Preparation
Alizarin Red S Solution:
- Alizarin Red S: 2 g
- Distilled water: 100 mL
Mix well and adjust the pH to 4.1-4.3 using 10% ammonium hydroxide. The pH is critical. Prepare the solution fresh or check the pH before use if the solution is more than one month old.
Histological Staining Procedure
- Deparaffinize the slides and bring them to distilled water.
- Stain the slides with Alizarin Red S solution for 30 seconds to 5 minutes while observing the reaction microscopically. The supplied procedure notes that approximately 2 minutes will usually produce orange-red calcium staining.
- Shake off excess dye and blot the tissue sections.
- Dehydrate in 100% acetone for 20 dips, followed by 20 dips in a 1:1 acetone-xylene solution.
- Clear in xylene and mount using a synthetic mounting medium.
Expected Result: Calcium deposits, except oxalate, appear orange-red. The resulting precipitate is birefringent.
Specificity note: The reaction is not strictly specific for calcium. Magnesium, manganese, barium, strontium, and iron may interfere under some conditions.
Safety: Review the applicable safety data sheets and follow institutional laboratory safety procedures when handling Alizarin Red S, acetone, and xylene.
Documents
The posted sample COA does not contain a lot number or lot-specific test results. Contact Biofargo to request documentation for the applicable product lot.
FAQ
What is AD0144 Alizarin Red S supplied as?
AD0144 is supplied as 25 g of indicator-grade Alizarin Red S powder. It is not a ready-to-use staining solution.
What application is described in the supplied product information?
The supplied product information describes the use of Alizarin Red S to identify calcium in neutral buffered formalin-fixed or alcoholic formalin-fixed, paraffin-embedded tissue sections.
How does Alizarin Red S staining identify calcium?
Calcium forms an Alizarin Red S-calcium complex through a chelation process. Under the supplied procedure, calcium deposits, except oxalate, appear orange-red, and the resulting precipitate is birefringent.
How is the Alizarin Red S staining solution prepared?
Dissolve 2 g of Alizarin Red S in 100 mL of distilled water. Mix well and adjust the pH to 4.1-4.3 using 10% ammonium hydroxide.
Why is the staining solution pH important?
The supplied procedure identifies pH 4.1-4.3 as critical for staining. Prepare the solution fresh or check the pH before use if the solution is more than one month old.
Is the Alizarin Red S staining reaction specific only to calcium?
No. The supplied product information states that the reaction is not strictly specific for calcium. Magnesium, manganese, barium, strontium, and iron may interfere, although these elements do not usually occur in sufficient concentrations to interfere with staining.
What positive control is recommended?
The supplied procedure recommends a mouse embryo or a known calcium-containing tissue section as a positive control.
How should AD0144 Alizarin Red S powder be stored?
Store at room temperature with the container tightly closed in a dry, well-ventilated place.
Is the posted COA lot-specific?
The posted document is a sample COA without a lot number or lot-specific test results. Contact Biofargo to request documentation for the applicable product lot.
Is this product intended for clinical or therapeutic use?
No. This product is intended for laboratory research use only and is not intended for human or animal drug use.
For laboratory research use only. Not for human or animal drug use.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
