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Description
Bio Basic’s Lysozyme for Molecular Biology is a high-purity, triple-crystallized enzyme designed for efficient cell wall lysis, particularly in Gram-positive bacteria. With a protein content of ≥90% and specific activity ≥20,000 units per milligram, this lyophilized preparation is ideal for DNA extraction, structural biology, and proteomics workflows. The enzyme hydrolyzes the β(1→4) glycosidic bonds between N-acetylmuramic acid and N-acetyl-D-glucosamine in bacterial cell walls, and is also effective on chitosan oligomers.
Specifications
| Grade | Biotech |
| CAS | 12650-88-3 |
| Appearance | White to off-white granulate |
| Solubility (2%-aqua at 640nm) | ≥95% |
| Purity on dry basis | ≥95% |
| Activity in FIP Units/mg protein | ≥39000 |
| pH (2%-aqua) | 3.0-3.6 |
| Water content | ≤6% |
| Residue on ignition | ≤1.5% |
| Storage | -20℃ |
Applications
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Bacterial cell lysis for plasmid DNA extraction
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Structural and functional studies of proteins
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Standard protein for mass spectrometry calibration
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Compatible with boiling lysis protocols
Solubility
Soluble at 10 mg/mL in distilled water; forms a clear or slightly hazy solution. Best reconstituted in 10 mM Tris-HCl, pH 8.0.
Storage
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Lyophilized: Stable for ≥4 years at -20°C
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Reconstituted (pH 4–5): Stable for weeks at 2–8°C
Documents
Disclaimer: For laboratory research use only.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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