GFP-Expressing Rabbit Primary Ovarian Microvascular Endothelial Cells (0.5x10^6)

Description

In vascular biology and microcirculation research, conventional primary endothelial cell models often lack real-time traceability and fluorescence stability, making it difficult to study dynamic processes such as endothelial migration, angiogenesis, and barrier permeability under physiological or pathological conditions. These limitations are especially significant in ovarian microvascular research, where subtle endothelial responses are critical for understanding tissue-specific vascular regulation.

This GFP-expressing rabbit ovarian microvascular endothelial cell model provides a stable fluorescent system derived from primary ovarian microvascular endothelium. Cells are engineered via lentiviral GFP transduction, producing strong green fluorescence under blue-light excitation, enabling real-time visualization of endothelial behavior including migration, network formation, and vascular remodeling.

Validated by DiI-Ac-LDL uptake functional assay, this model retains key endothelial characteristics and is widely used for vascular biology research, angiogenesis studies, endothelial barrier function assays, and live cell imaging applications in microvascular systems.

Features

• GFP-expressing rabbit ovarian microvascular endothelial cells

• Stable fluorescent vascular tracking system for live imaging

• Validated endothelial phenotype via DiI-Ac-LDL uptake assay

• Gelatin-coated culture compatibility for stable adhesion

• Suitable for angiogenesis and migration assays

• Limited passage primary cell model (3–5 passages)

Specifications

Applications

• Ovarian microvascular biology research

• Endothelial barrier function studies

• Angiogenesis and vascular remodeling assays

• Live cell imaging and fluorescent tracking

• Microcirculation and vascular permeability studies

• Cardiovascular and vascular disease modeling

• Drug screening on endothelial systems

• Inflammation-induced vascular response studies

Fluorescent GFP Imaging System

The GFP labeling system enables high-resolution real-time imaging of endothelial cell dynamics. Under blue-light excitation, cells emit strong green fluorescence suitable for quantitative analysis of:

• Cell migration behavior

• Angiogenic network formation

• Endothelial barrier integrity changes

• Cell-cell interaction dynamics

Cell Culture System

Gelatin-coated surfaces are recommended to support endothelial adhesion, morphology stability, and physiological behavior during long-term imaging experiments.

• Coating type: gelatin-based extracellular matrix mimic

• Function: enhances endothelial attachment and spreading

• Compatibility: standard endothelial growth medium systems

Storage & Handling

• Store in liquid nitrogen (-180°C) for long-term preservation

• Do not store at -80°C or -20°C

• Avoid repeated freeze-thaw cycles

• Handle under sterile biosafety conditions

• Protect GFP fluorescence from excessive light exposure

Documents

N-6090GFP Datasheet

Cell Culture Protocol

Frequently Asked Questions