GFP-Expressing Porcine Primary Pulmonary Vein Endothelial Cells (0.5x10^6)

Description

In vascular biology and pulmonary circulation research, traditional primary endothelial cell models often suffer from limited traceability, low imaging contrast, and difficulty in real-time tracking of cellular behavior during angiogenesis, permeability assays, and migration studies. These limitations create challenges in studying dynamic endothelial responses under physiological and pathological conditions such as vascular inflammation, atherosclerosis, and pulmonary vascular remodeling.

This GFP-expressing porcine pulmonary vein endothelial cell model provides a stable fluorescent vascular system derived from primary pulmonary vein endothelium. Cells are engineered via lentiviral GFP transduction, enabling strong green fluorescence under blue-light excitation for real-time visualization of endothelial behavior, including migration, alignment, and barrier dynamics.

Validated through DiI-Ac-LDL uptake functional assay, this model retains key endothelial characteristics and is suitable for vascular biology research, angiogenesis studies, endothelial barrier function analysis, and live cell imaging applications.

Features

• GFP-expressing porcine pulmonary vein endothelial cells (fluorescent vascular model)

• Stable lentiviral GFP labeling for live cell imaging

• Validated endothelial phenotype via DiI-Ac-LDL uptake assay

• Gelatin-coated surface compatible cell system

• Suitable for real-time tracking of endothelial migration and angiogenesis

• Cryopreserved primary cell format for research applications

Specifications

Applications

• Vascular endothelial cell biology research

• Live cell imaging and fluorescence tracking

• Angiogenesis and endothelial migration assays

• Endothelial barrier function studies

• Pulmonary vascular remodeling research

• Atherosclerosis and cardiovascular disease models

• Drug screening on vascular endothelium

• Inflammation-induced endothelial response studies

Fluorescent GFP System

The GFP reporter enables high-resolution visualization of endothelial dynamics in real time. Under blue-light excitation, cells emit strong green fluorescence, allowing quantitative tracking of:

• Cell migration behavior

• Endothelial network formation

• Cell-cell interaction dynamics

• Barrier integrity changes

Cell Culture System

Gelatin-coated substrates are recommended to enhance endothelial adhesion and maintain physiological morphology during expansion and imaging experiments.

• Coating material: gelatin-based extracellular matrix mimic

• Purpose: improve endothelial attachment and stability

• Compatibility: standard endothelial growth medium systems

Storage & Handling

• Store in liquid nitrogen (-180°C) for long-term preservation

• Do not store at -80°C or -20°C

• Avoid repeated freeze-thaw cycles

• Handle under sterile biosafety conditions

• Minimize light exposure to preserve GFP signal integrity

Documents

P-6060GFP Datasheet

Cell Culture Protocol

Frequently Asked Questions