0 item(s)
View cart
You have no items in your shopping cart.
$1,500.00
Sku: P-6023GFP
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by July 11, 2026
Product Notice
- Made to order product – estimated lead time: 1–2 months
- Primary cell limitation: non-immortalized system with restricted expansion capacity
- Passaging: not recommended by manufacturer; cells are intended for direct experimental use after recovery
Description
In blood-brain barrier (BBB) and neurovascular research, primary brain microvascular endothelial cells are essential for modeling endothelial transport and barrier integrity. However, conventional non-labeled primary cells often limit real-time visualization of dynamic processes such as endothelial migration, tight junction regulation, and permeability changes under physiological or disease conditions.
The GFP-expressing porcine primary brain microvascular endothelial cells (P-6023GFP) provide a physiologically relevant in vitro BBB model derived from porcine brain tissue. These primary endothelial cells are stably labeled via lentiviral GFP transduction, enabling bright green fluorescence for real-time tracking of cellular behavior during vascular remodeling and barrier function studies.
Functionality is confirmed by DiI-Ac-LDL uptake assay, demonstrating endothelial identity and metabolic activity. This model is widely used in blood-brain barrier research, neurovascular biology, CNS drug delivery studies, endothelial permeability assays, and live-cell imaging applications.
Features
-
Primary porcine brain microvascular endothelial cells with stable GFP expression
-
Physiologically relevant in vitro blood-brain barrier (BBB) model
-
Bright fluorescence enables real-time live cell imaging and tracking
-
Validated endothelial function via DiI-Ac-LDL uptake assay
-
Suitable for permeability, transport, and neurovascular studies
-
Limited passage primary system (3–5 passages recommended)
-
Compatible with gelatin-coated endothelial culture systems
Specifications
| Cell Type | Porcine Brain Microvascular Endothelial Cells |
| Genetic Label | GFP (Green Fluorescent Protein), lentiviral stable expression |
| Functional Validation | DiI-Ac-LDL uptake assay |
| Model Type | Blood-Brain Barrier (BBB) in vitro model |
| Passage Range | 3–5 passages recommended |
| Storage | Liquid nitrogen (-180°C) |
| Shipping | Dry ice (cryopreserved vial) |
| Catalog No. | P-6023GFP |
Applications
-
Blood-brain barrier (BBB) permeability assays
-
CNS drug delivery and transport studies
-
Neurovascular biology and microcirculation research
-
Endothelial barrier integrity and tight junction analysis
-
Live-cell fluorescence imaging of endothelial dynamics
-
Angiogenesis and vascular remodeling studies
-
Drug screening for CNS-targeted therapeutics
-
Pathophysiological modeling of neurovascular disorders
GFP Fluorescent Imaging System
The integrated GFP labeling system enables high-resolution real-time visualization of endothelial cell behavior under blue-light excitation. This allows direct observation of:
-
Endothelial migration and alignment
-
Barrier integrity changes under stimulation
-
Microvascular network formation
-
Cell-cell interaction dynamics in BBB models
Cell Culture System
These primary endothelial cells are optimized for standard endothelial culture workflows using gelatin-coated flasks and endothelial growth medium systems.
-
Recommended coating: gelatin-based extracellular matrix
-
Culture system: endothelial growth medium (complete system)
-
Subculture ratio: 1:2 at ~90% confluence
-
Recommended handling: sterile biosafety conditions
Storage & Handling
-
Store cryovials in liquid nitrogen (-180°C) immediately upon arrival
-
Do not store at -80°C or -20°C
-
Avoid repeated freeze–thaw cycles
-
Use aseptic techniques during all handling steps
-
Protect GFP fluorescence from prolonged light exposure
Documents
Frequently Asked Questions
What is GFP-expressing porcine brain microvascular endothelial cells used for?
This cell model is primarily used for blood-brain barrier (BBB) research, CNS drug delivery studies, endothelial permeability assays, and neurovascular biology research.
Why use porcine brain microvascular endothelial cells for BBB models?
Porcine brain endothelial cells provide high physiological relevance due to similarities in brain microvascular structure and barrier function compared to human neurovascular systems.
What is the advantage of GFP labeling in this cell model?
GFP enables real-time fluorescence tracking of endothelial behavior, allowing researchers to observe migration, barrier changes, and vascular network formation without additional staining.
How is endothelial identity confirmed?
Endothelial phenotype is validated using DiI-Ac-LDL uptake assay, which confirms functional lipid uptake characteristic of endothelial cells.
What are the recommended storage conditions?
Cells must be stored in liquid nitrogen (-180°C). Storage at -80°C or -20°C is not recommended due to reduced viability and fluorescence stability.
Can these cells be used for permeability and drug transport studies?
Yes. These cells are widely used in BBB permeability assays and CNS drug transport studies to evaluate compound penetration across the blood-brain barrier.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
