GFP-Expressing Canine Primary Ovarian Microvascular Endothelial Cells (0.5x10^6)

Description

In ovarian vascular biology and angiogenesis research, conventional endothelial cell lines often fail to replicate the complex microvascular environment required for studying follicular development, endocrine regulation, and reproductive tissue remodeling. This limitation restricts accurate modeling of ovarian microcirculation and vascular signaling pathways.

The GFP-expressing canine primary ovarian microvascular endothelial cells (D-6090GFP) provide a physiologically relevant primary endothelial model derived from beagle ovarian tissue. These cells maintain native microvascular characteristics and are stably labeled with green fluorescent protein (GFP), enabling real-time visualization of endothelial dynamics during angiogenesis and vascular remodeling.

Functional endothelial identity is confirmed by DiI-Ac-LDL uptake assay, supporting lipid uptake activity and endothelial phenotype stability. This model is widely used in ovarian angiogenesis research, reproductive vascular biology, endothelial permeability assays, and microcirculation studies.

Features

• Primary canine ovarian microvascular endothelial cells with stable GFP expression

• Physiologically relevant ovarian angiogenesis model

• Real-time fluorescence imaging for vascular dynamics tracking

• Validated endothelial function via DiI-Ac-LDL uptake assay

• Suitable for angiogenesis, permeability, and vascular remodeling studies

• Limited passage primary endothelial system

• Compatible with gelatin-coated endothelial culture conditions

Specifications

Applications

• Ovarian angiogenesis research models

• Reproductive vascular biology studies

• Endothelial permeability and barrier function assays

• Follicular microcirculation research

• Drug screening for reproductive system targeting

• Hormonal regulation of vascular networks

• Inflammation-related vascular response studies

• Live-cell imaging of endothelial migration and remodeling

Ovarian Angiogenesis & Microvascular System

This GFP-labeled endothelial model enables real-time analysis of ovarian vascular remodeling and angiogenic signaling. It is particularly suitable for:

• Endothelial sprouting and vessel formation studies

• Microvascular permeability assays

• Follicle-associated vascular development

• Reproductive tissue vascular interaction studies

Cell Culture System

• Recommended coating: gelatin-based extracellular matrix

• Endothelial growth medium compatible system

• Supports short-term primary culture workflows

• Recommended early-passage experimental use

Storage & Handling

• Store in liquid nitrogen (-180°C) immediately upon arrival

• Do not store at -80°C or -20°C

• Avoid repeated freeze–thaw cycles

• Use sterile biosafety techniques

• Protect GFP fluorescence from prolonged light exposure

Documents

D-6090GFP Datasheet

Cell Culture Protocol

Frequently Asked Questions