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$1,300.00
Sku: D-6053GFP
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by July 13, 2026
Product Notice
- Made to order product – estimated lead time: 1–2 months
- Primary cell limitation: limited expansion capacity typical of primary alveolar epithelial cells
- Passaging: not recommended for long-term culture; use within validated early passages
Description
In respiratory and pulmonary research, traditional epithelial cell lines often fail to reproduce the physiological complexity of alveolar barrier function, especially in studies involving air-liquid interface (ALI) culture, inhalation toxicity, and lung injury modeling. This limitation makes it difficult to accurately simulate in vivo lung epithelial responses.
The GFP-expressing canine primary alveolar epithelial cells (D-6053GFP) provide a physiologically relevant primary lung epithelial model derived from beagle alveolar tissue. These cells retain native epithelial characteristics and are stably labeled with green fluorescent protein (GFP), enabling real-time visualization of cellular dynamics in lung barrier systems.
This model is validated by ZO-1 tight junction expression, confirming epithelial barrier integrity. It is widely used in alveolar epithelial barrier research, ALI (air-liquid interface) models, pulmonary fibrosis studies, ARDS research, and inhalation toxicity screening.
Features
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Primary canine alveolar epithelial cells with stable GFP expression
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Physiologically relevant lung epithelial barrier model
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Suitable for air-liquid interface (ALI) culture systems
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Validated by ZO-1 tight junction protein expression
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Supports live cell imaging and fluorescent tracking
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Limited passage primary cell system
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Compatible with standard epithelial culture conditions
Specifications
| Cell Type | Canine Primary Alveolar Epithelial Cells |
| Species | Beagle Dog |
| Genetic Label | GFP (Green Fluorescent Protein), lentiviral expression |
| Functional Validation | ZO-1 tight junction immunofluorescence |
| Model Type | Lung epithelial barrier / ALI model |
| Storage | Liquid nitrogen (-180°C) |
| Shipping | Dry ice (cryopreserved vial) |
| Catalog No. | D-6053GFP |
Applications
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Air-liquid interface (ALI) lung epithelial modeling
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Pulmonary fibrosis mechanism research
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Acute lung injury (ALI/ARDS) in vitro models
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Inhalation toxicity and nanomaterial exposure studies
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Lung epithelial barrier integrity assays
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Drug screening for respiratory system therapeutics
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Airway inflammation and epithelial repair studies
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Live-cell imaging of alveolar epithelial dynamics
Alveolar Epithelial Barrier System
This GFP-labeled model enables real-time visualization of alveolar epithelial behavior under physiological or injury conditions. It is particularly suitable for:
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Air-liquid interface (ALI) differentiation studies
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Tight junction formation and disruption analysis
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Epithelial wound repair and regeneration
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Barrier permeability and transport studies
Cell Culture System
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Recommended coating: gelatin-based extracellular matrix
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Suitable for epithelial growth medium systems
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Supports ALI culture adaptation workflows
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Recommended for early-passage experimental use
Storage & Handling
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Store in liquid nitrogen (-180°C) immediately upon arrival
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Do not store at -80°C or -20°C
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Avoid repeated freeze–thaw cycles
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Use sterile biosafety techniques during handling
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Minimize light exposure to preserve GFP fluorescence
Documents
Frequently Asked Questions
What are GFP-expressing canine primary alveolar epithelial cells used for?
They are used for lung epithelial barrier research, ALI (air-liquid interface) models, pulmonary fibrosis studies, and inhalation toxicity testing.
Why are alveolar epithelial cells important in lung research?
Alveolar epithelial cells form the primary barrier for gas exchange and are essential for studying lung injury, inflammation, and respiratory disease mechanisms.
What is the advantage of GFP labeling in this model?
GFP enables real-time visualization of epithelial cell behavior, including migration, barrier formation, and injury response without additional staining.
Can this model be used for ALI (air-liquid interface) studies?
Yes, this model is compatible with ALI systems and is widely used for differentiation and epithelial barrier function studies.
How is epithelial integrity validated?
Epithelial phenotype is confirmed by ZO-1 tight junction expression, indicating functional barrier formation.
What diseases can this model simulate?
It is widely used in pulmonary fibrosis, acute lung injury (ALI/ARDS), airway inflammation, and inhalation toxicity research.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
