CMRL 1066(10X)

Specification

Application

• General cell culture:

  Supports the growth and maintenance of a variety of human and animal cell lines, including primary cells, fibroblasts, and tumor cell cultures.

• Tissue and organ culture:

  Due to its inclusion of nucleosides and coenzymes (such as NAD, NADP, and Coenzyme A), CMRL-1066 is suitable for short-term maintenance of mammalian tissue fragments or organ explants in vitro.

• Low-serum or serum-free systems:

  The presence of ascorbic acid, glutathione, cholesterol, and Tween 80 helps maintain membrane stability and cellular vitality under low-serum conditions. Hence, it is frequently used in the development of serum-free or chemically defined culture systems.

• Host–pathogen co-culture studies:

  As a nutritionally rich basal medium, CMRL-1066 is often employed for the co-culture of cell-dependent microorganisms such as Mycoplasma, Rickettsia, or certain viruses.

• Cell recovery and pre-freezing maintenance:

  With multiple antioxidant and metabolic support components (e.g., glucose, niacinamide, FAD, glutathione), it is also used for short-term recovery culture prior to cryopreservation or thawing.

Features

• Enhanced Lipid Supplementation:

  Incorporation of soluble lipid components improves cellular and mycoplasma metabolism, optimizes membrane composition, and enhances stress resistance—resulting in superior culture performance.

• Stable Physiological Buffering:

  Formulated with 20 mM HEPES, maintaining pH precisely at 7.4 to ensure consistent environmental stability throughout incubation and handling.

• Serum Protein Enrichment:

  Addition of defined serum proteins enhances culture stability and reinforces overall environmental consistency for reproducible results.

• Simplified, Indicator-Free System:

  Sodium bicarbonate and phenol red have been removed to reduce variability and eliminate potential optical or chemical interferences during sensitive assays.

• Optimized Amino Acid Profile:

  L-glutamine and L-cysteine are excluded to minimize degradation-related byproducts and maintain long-term formulation stability.

• Light-Protective Packaging:

  Supplied in amber bottles to preserve the integrity of light-sensitive components and ensure sustained biochemical activity during storage.

Documents

M-CMRL-10 SDS