C57BL/6 Mouse Skeletal Muscle Cells

Description

C57BL/6 Mouse Primary Skeletal Muscle Cells from Cell Biologics are isolated from the skeletal muscle tissues of pathogen-free laboratory C57BL/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. C57BL/6 Mouse Primary Skeletal Muscle Cells are characterized by immunofluorescent staining with antibodies of myosin and/or actinin. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.

Suggested Medium

Skeletal Muscle Cell Medium /w Kit – 500 ML

Catalog No: M1261

(500 ml Basal medium with M1261-Kit)

Storage

Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

C57-6167

Cell-Culture-Protocol-CB

1708462493

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.