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$20.00
Sku: 6016011
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by August 18, 2026
Description
Selecting an RPMI-1640 cell culture medium requires confirming whether the formulation contains L-glutamine, phenol red, sodium bicarbonate, HEPES, sodium pyruvate, or antibiotics. This RPMI-1640 Medium contains L-glutamine, phenol red, and sodium bicarbonate, while HEPES, sodium pyruvate, and antibiotics are not included.
RPMI-1640 Medium was developed by Moore et al. at Roswell Park Memorial Institute. It was originally developed to culture human leukemic cells in suspension and as a monolayer. It has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and hybridomas.
This RPMI-1640 cell culture medium requires supplementation and is commonly supplemented with 10% fetal bovine serum (FBS).
Formulation overview: With L-glutamine, phenol red, and sodium bicarbonate; without HEPES, sodium pyruvate, or antibiotics.
Specifications
| Attribute | Specification |
|---|---|
| Product Name | RPMI-1640 Medium |
| Catalog Number | 6016011 |
| Volume | 500 mL |
| Supplementation | Requires supplementation; commonly supplemented with 10% fetal bovine serum (FBS) |
| L-Glutamine | Included, 300 mg/L |
| Phenol Red | Included, 5 mg/L |
| Antibiotics | Not included |
| Sodium Pyruvate | Not included |
| Sodium Bicarbonate | Included, 2000 mg/L |
| HEPES | Not included |
| pH | 7.0–7.6 |
| Osmolality | 265–305 mOsmol/kg |
| Endotoxin | <0.3 EU/mL |
| Storage | 2–8°C, protected from light |
| Shelf Life | 12 months |
Features
- Contains L-glutamine at 300 mg/L.
- Contains phenol red at 5 mg/L.
- Contains sodium bicarbonate at 2000 mg/L.
- Does not contain HEPES, sodium pyruvate, or antibiotics.
- Specified pH range of 7.0–7.6.
- Specified osmolality range of 265–305 mOsmol/kg.
- Endotoxin specification of less than 0.3 EU/mL.
- Supplied in a 500 mL format.
Applications
This RPMI-1640 Medium is suitable for the mammalian cell culture applications and cell types identified in the product information:
- Suspension culture of human leukemic cells
- Monolayer culture of human leukemic cells
- HeLa cell culture
- Jurkat cell culture
- MCF-7 cell culture
- PC12 cell culture
- PBMC culture
- Astrocyte culture
- Hybridoma culture
- Culture of the listed mammalian cell types
Formulation
The following RPMI-1640 formulation components are listed in milligrams per liter (mg/L).
Amino Acids
| Component | Concentration (mg/L) |
|---|---|
| Glycine | 10 |
| L-Arginine (free base) | 200 |
| L-Asparagine (anhyd.) | 50 |
| L-Aspartic Acid | 20 |
| L-Cystine·2HCl | 65 |
| L-Glutamic Acid | 20 |
| L-Glutamine | 300 |
| L-Histidine (free base) | 15 |
| L-Hydroxyproline | 20 |
| L-Isoleucine | 50 |
| L-Leucine | 50 |
| L-Lysine·HCl | 40 |
| L-Methionine | 15 |
| L-Phenylalanine | 15 |
| L-Proline | 20 |
| L-Serine | 30 |
| L-Threonine | 20 |
| L-Tryptophan | 5 |
| L-Tyrosine·2Na·2H₂O | 29 |
| L-Valine | 20 |
Vitamins
| Component | Concentration (mg/L) |
|---|---|
| D-Biotin | 0.2 |
| Choline Chloride | 3 |
| Folic Acid | 1 |
| myo-Inositol | 35 |
| Niacinamide | 1 |
| p-Amino Benzoic Acid | 1 |
| D-Pantothenic Acid·1/2 Ca | 0.25 |
| Pyridoxal·HCl | 1 |
| Riboflavin | 0.2 |
| Thiamine·HCl | 1 |
| Vitamin B-12 | 0.005 |
Inorganic Salts
| Component | Concentration (mg/L) |
|---|---|
| Ca(NO₃)₂·4H₂O | 100 |
| MgSO₄ (anhyd.) | 48.84 |
| KCl | 400 |
| NaHCO₃ | 2000 |
| NaCl | 6000 |
| NaH₂PO₄ (anhyd.) | 800 |
Other Components
| Component | Concentration (mg/L) |
|---|---|
| D-Glucose | 2000 |
| Glutathione (reduced) | 1 |
| Phenol Red·Na | 5 |
Storage and Handling
Storage
Store at 2–8°C and protect from light.
The stated shelf life is 12 months.
Handling Notes
- Avoid repeated freezing and thawing.
- Use aseptic technique during handling.
Frequently Asked Questions
Does this RPMI-1640 Medium contain L-glutamine?
Yes. This RPMI-1640 formulation contains L-glutamine at 300 mg/L.
Does this RPMI-1640 Medium contain phenol red?
Yes. The formulation contains phenol red sodium salt at 5 mg/L.
Does this RPMI-1640 Medium contain sodium bicarbonate?
Yes. The formulation contains sodium bicarbonate at 2000 mg/L.
Does this RPMI-1640 formulation contain HEPES?
No. HEPES is not included in this RPMI-1640 Medium.
Does the medium contain sodium pyruvate or antibiotics?
No. Sodium pyruvate and antibiotics are not included in this formulation.
Does RPMI-1640 Medium require FBS supplementation?
The product information states that RPMI-1640 Medium requires supplementation and is commonly supplemented with 10% fetal bovine serum (FBS).
Which cells are listed as suitable for this RPMI-1640 Medium?
The product information lists HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and hybridomas.
Is this RPMI-1640 Medium suitable for suspension cell culture?
The product information states that RPMI-1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
What are the specified pH and osmolality ranges?
The specified pH range is 7.0–7.6, and the specified osmolality range is 265–305 mOsmol/kg.
What is the endotoxin specification?
The stated endotoxin specification is less than 0.3 EU/mL.
How should RPMI-1640 Medium be stored?
Store the medium at 2–8°C and protect it from light. The stated shelf life is 12 months.
Documents
When can I expect my order to ship?
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Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
