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$28.00
Sku: A00268-1L
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by July 14, 2026
Description
In nucleic acid electrophoresis workflows, inconsistent buffer preparation can lead to poor band resolution, variable migration patterns, excessive heating, or unreliable DNA and RNA separation. Laboratories running agarose gel electrophoresis or polyacrylamide gel electrophoresis need a dependable electrophoresis buffer that is easy to dilute, consistent between runs, and suitable for routine molecular biology research.
10× TBE Buffer (Tris-Borate-EDTA) is a ready-to-dilute liquid concentrate formulated with Tris, boric acid, EDTA, and water. It is designed for preparation of 1× TBE working solution used in DNA and RNA electrophoresis workflows, including agarose gel systems and polyacrylamide gel systems.
This biotech grade TBE electrophoresis buffer is supplied in a convenient 1000 mL format, helping reduce manual weighing, mixing, and buffer preparation time while supporting consistent electrophoresis performance in laboratory research applications.
Specification
| Product Name | 10× TBE Buffer (Tris-Borate-EDTA) |
| Product Code | A00268 |
| Concentration | 10× |
| Form | Liquid solution |
| Grade | Biotech |
| Composition | Tris, boric acid, EDTA, and water |
| Product Volume | 1000 mL |
| UNSPSC Category | Electrophoresis Buffers |
| Storage | 18 to 25 °C |
| Recommended Use | For laboratory research use only |
Features
- 10× concentrated format: Designed for convenient dilution to working concentration before electrophoresis use.
- Tris-Borate-EDTA formulation: Contains Tris, boric acid, and EDTA in an aqueous buffer solution.
- Electrophoresis buffer category: Suitable for laboratory workflows requiring TBE running buffer systems.
- Supports routine nucleic acid analysis: Applicable to DNA and RNA gel electrophoresis workflows.
- Convenient 1000 mL bottle: Reduces repeated manual buffer preparation for routine lab use.
- Biotech grade quality: Intended for research laboratories requiring consistent buffer preparation.
Applications
- Agarose gel electrophoresis: Used to prepare TBE buffer systems for routine DNA fragment separation and analysis.
- Polyacrylamide gel electrophoresis: Suitable for workflows requiring Tris-Borate-EDTA buffer in PAGE-based nucleic acid separation.
- DNA electrophoresis: Supports molecular biology workflows involving plasmid DNA, PCR products, restriction digest products, and other DNA samples.
- RNA electrophoresis: Can be used in research workflows requiring TBE buffer conditions for RNA sample analysis.
- Running buffer preparation: Designed for dilution from 10× concentrate to 1× TBE working solution before electrophoresis.
- Gel preparation buffer: May be used when preparing gel systems that require TBE buffer compatibility.
- Nucleic acid gel electrophoresis workflows: Appropriate for academic, biotech, and molecular biology laboratories performing routine sample separation.
- Teaching and training laboratories: Useful for standard electrophoresis demonstrations where a ready-to-dilute TBE buffer concentrate is preferred.
Dilution & Handling Notes
Dilute the 10× TBE buffer concentrate to the required working concentration using suitable laboratory-grade water according to your electrophoresis protocol. For consistent gel performance, prepare fresh working buffer as needed and use the same buffer system for both gel preparation and electrophoresis running conditions when required by the protocol.
Keep the container tightly closed when not in use. Avoid introducing pipette tips, used buffer, gel residues, or other contaminants into the original bottle.
Stability & Storage
Store at 18–25 °C.
Keep container tightly closed in a dry and well-ventilated place.
Avoid contamination during handling and use.
Documents
Frequently Asked Questions
What is 10× TBE Buffer used for?
10× TBE Buffer is a Tris-Borate-EDTA electrophoresis buffer concentrate used to prepare working buffer for nucleic acid electrophoresis, including DNA and RNA separation in agarose gel and polyacrylamide gel systems.
Can this TBE buffer be used for agarose gel electrophoresis?
Yes. This 10× TBE buffer can be diluted to the working concentration required by your protocol and used in agarose gel electrophoresis workflows for DNA or RNA sample analysis.
Is TBE buffer suitable for polyacrylamide gel electrophoresis?
Yes. Tris-Borate-EDTA buffer is commonly used in polyacrylamide gel electrophoresis workflows where a TBE buffer system is specified for nucleic acid separation.
How do I prepare 1× TBE working solution from 10× TBE Buffer?
Dilute the 10× TBE concentrate according to your laboratory protocol to obtain the desired working concentration. For a standard 1× solution, laboratories typically dilute one part 10× concentrate with nine parts suitable laboratory-grade water.
Can TBE buffer be used as both running buffer and gel preparation buffer?
Yes, when required by the electrophoresis protocol, diluted TBE working buffer may be used for both gel preparation and as the running buffer. Using the same buffer system helps maintain consistent ionic conditions during electrophoresis.
What is the difference between TBE buffer and TAE buffer?
TBE buffer contains Tris, borate, and EDTA, while TAE buffer contains Tris, acetate, and EDTA. TBE is often selected for nucleic acid electrophoresis workflows that require strong buffering capacity and stable migration conditions. The best choice depends on the sample type, gel format, downstream workflow, and laboratory protocol.
Why use a ready-to-dilute 10× TBE buffer instead of preparing buffer from powder?
A liquid 10× TBE buffer concentrate reduces weighing and mixing steps, saves preparation time, and helps support more consistent electrophoresis workflows compared with manually preparing buffer from individual components.
How should this 10× TBE Buffer be stored?
Store this product at 18–25 °C. Keep the bottle tightly closed, avoid contamination, and follow standard laboratory chemical handling practices.
Is this product intended for diagnostic or therapeutic use?
No. This 10× TBE Buffer is intended for laboratory research use only and is not for human or animal drug use.
For laboratory research or industrial use only.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
