Bingene Dye for Nucleic Acid Amplification

Product Description

Bingene Dye is a green fluorescent nucleic acid dye that is non-fluorescent by itself, but becomes fluorescent upon binding to dsDNA. It has the following features:

• Lower fluorescent background.

• Higher fluorescence intensity.

• Less inhibitory than SYBR Green I and less likely to cause non-specific amplification.

• Environmentally safe, non-mutagenic, and non-cytotoxic. Unsurpassed thermal stability, chemical stability, and photostability.

• Compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region.

• Amplification reaction can be monitored using existing optical setting for SYBR Green I or FAM on any commercial real-time PCR cycler.

Kit Contents

Storage and Handling

Store the product at 2-25℃. The product is stable for at least one year from date of receipt.

Procedures

1. Set up the nucleic acid amplification reaction as usual，add 20×Bingene Dye at a ratio of 20:1 (for a reaction of 20mL final volume, add 1 mL Bingene Dye)，add ddH2O to the final volume of choice.

2. Perform amplification on a thermocycling fluorometer and record the fluorescence signal at the annealing or extension step for PCR or collect signal in every minute for isothermal amplification.

Note: When using ABI Sequence Detection Systems, make sure to select NONE for the passive reference under the tab WELL INSPECTOR