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$10.00
Sku: Y02001N-10ug
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by June 06, 2026
Description
Recombinant Human Interleukin-2 (IL-2) is a cytokine that plays a central role in the regulation of immune responses. It is primarily produced by activated T lymphocytes and promotes the proliferation, differentiation, and survival of T cells, B cells, and natural killer (NK) cells. This recombinant IL-2 protein is expressed in CHO cells and corresponds to the mature human IL-2 sequence (Ala21–Thr153), ensuring proper folding and post-translational modifications. With high purity (≥95%) and strong bioactivity (≥1 × 107 IU/mg), this product is suitable for a wide range of immunological and cell biology studies. The protein is supplied in a tag-free, lyophilized form, making it convenient for reconstitution and downstream applications.
Specifications
| Product Details | |
|---|---|
| Cat. No. | Y02001N |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤1 EU/mg by the LAL method. |
| Bioactivity | ≥1 x 107 IU/mg. Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. |
| Accession | P60568-1 |
| Species | Human |
| Expression System | CHO |
| Mol. Weight | 15.4 kDa |
| Expression Region | Ala21-Thr153 |
| Tag Info | Tag free |
| Formulation | Lyophilized |
| Buffer | PBS, 5% Mannitol and 0.01% Tween 80, pH7.4. |
| Size | 100μg/1mg |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: High Purity: This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring the reliability and reproducibility of experimental results.
- ✔ High Biological Activity: Exhibits a bioactivity of ≥1 × 10⁷ IU/mg, validated by CTLL-2 cell proliferation assay, ensuring strong and consistent performance in functional studies.
- ✔ Mammalian Expression System: Expressed in CHO cells to ensure proper protein folding and native post-translational modifications, enhancing biological relevance.
- ✔ Low Endotoxin: Endotoxin level is ≤1 EU/mg, making it suitable for sensitive cell-based assays and immune-related studies.
Applications
Hematology
Regulates hematopoietic balance under inflammation, favoring myeloid differentiation.
Immunology
Potent pro-inflammatory cytokine driving innate immunity and Th17 responses.
Cell Biology
Participates in inflammation signaling, cell activation, and death regulation.
Data Figures
Figure 1: Recombinant Human IL-2 (Cat. No. Y02001) stimulates proliferation of CTLL-2 cells. The specific bioactivity of Y02001 is ≥1 x 107 IU/mg.
Figure 2: Recombinant Human IL-2 (Cat. No. Y02001) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y02001 SDS-PAGE.
Figure 3: Highly consistent bioactivity between different production batches of Recombinant Human IL-2 (Cat. No. Y02001).
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
