DNA / RNA / Protein Quantification, 1–20 µL

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Description

Laboratories working with limited DNA, RNA or protein samples need rapid concentration measurements without consuming excessive material. The Biofargo Fluorometer X Series uses 1–20 µL of sample and completes each measurement in no more than five seconds.

Three configurations support different laboratory requirements. The single-sample Biofargo X1 measures DNA and RNA. The eight-sample X2 is designed for DNA measurement, while the eight-sample X3 supports DNA, RNA, ssDNA and protein measurement together with direct Fluorometer and Kinetics programs.

Specification

Model Catalog Number Throughput Supported Measurements Additional Programs
Biofargo X1 FS-M2001 1 sample DNA and RNA
Biofargo X2 FS-M2002 Up to 8 samples DNA only
Biofargo X3 FS-M2003 Up to 8 samples DNA, RNA, ssDNA and protein Fluorometer and Kinetics
Instrument Type Microvolume fluorometer
Sample Volume 1–20 µL
Measurement Time ≤5 seconds per sample
Optical System 460–480 nm excitation; 500–535 nm emission; SiPMT detector
Linear Range Five orders of magnitude
Power DC 5 V, 2 A; maximum 10 W
Dimensions / Weight 110 × 230 × 45.5 mm / approximately 0.5 kg
Operating Conditions 15–30°C; relative humidity ≤75%; indoor use

Documented Quantification Ranges

Model Program Detection Range
X1 dsDNA 0.01–1000 ng/µL
X1 RNA 0.25–1000 ng/µL
X3 dsDNA High Sensitivity 0.005–120 ng/µL
X3 dsDNA Broad Range 0.2–2000 ng/µL
X3 microRNA 0.05–100 ng/µL
X3 ssDNA / Oligo 0.05–200 ng/µL
X3 Protein Broad Range 100–2000 ng/µL

Features

  • Uses only 1–20 µL of sample for microvolume fluorescence measurements.
  • Completes each measurement in no more than five seconds.
  • X2 and X3 process as many as eight samples in one run.
  • Two-point calibration supports quantitative assay workflows.
  • Touchscreen operation supports assay selection, calibration, measurement and result review.
  • The X3 provides USB data export, direct fluorescence measurement and fluorescence kinetics programs.

Application

NGS Library Quantification

Quantification of DNA libraries before next-generation sequencing loading using a compatible fluorescence assay. The 1–20 µL sample-volume requirement helps conserve prepared NGS library material.

qPCR Template Quantification

Measurement of DNA or RNA template concentrations before qPCR or RT-qPCR setup. Quantification can support consistent template input across experimental reactions.

AAV DNA and mRNA Quantification

Fluorescence-based quantification of prepared AAV DNA or mRNA samples using a compatible assay kit. Select a Biofargo model that supports the required nucleic acid type.

Single-Cell Library Quantification

Microvolume quantification of limited single-cell library preparations before downstream processing. The X3 high-sensitivity dsDNA program has a documented concentration range of 0.005–120 ng/µL.

Teaching Laboratories

Suitable for instructional laboratory workflows involving fluorescence measurement, nucleic acid quantification, two-point calibration and result review. The touchscreen interface supports guided operation.

FAQ

What is the difference between the Biofargo X1, X2 and X3?

The X1 measures one DNA or RNA sample at a time. The X2 processes up to eight DNA samples per run. The X3 processes up to eight samples and supports DNA, RNA, ssDNA and protein measurement.

Which Biofargo model supports protein and ssDNA measurement?

Protein and ssDNA measurement are supported by the Biofargo X3. The X1 supports DNA and RNA, while the X2 is designed for DNA only.

How much sample is required?

The documented microvolume workflow requires 1–20 µL of sample.

How long does each fluorescence measurement take?

Each measurement is completed in no more than five seconds per sample.

Which models process multiple samples?

The Biofargo X2 and X3 process up to eight samples per run. The Biofargo X1 measures one sample at a time.

Can Biofargo measurement data be exported?

The Biofargo X3 supports export of selected experimental records after a USB flash drive is inserted.

For laboratory research use only.

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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