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$35.00
Sku: SD7020-250g
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by July 13, 2026
Description
In molecular biology and recombinant protein research, inconsistent bacterial growth, low phage yield, and poor plasmid amplification are often caused by nutrient-limited or low-quality culture media. Researchers working with E. coli expression systems and filamentous phage display require a richer and more stable alternative to standard LB medium.
2×YT Agar (2xYT Medium) is a nutritionally enriched microbiological growth medium optimized for high-density E. coli cultivation and efficient filamentous phage (M13) propagation. Compared with standard LB medium, 2×YT provides higher levels of peptides, amino acids, vitamins, and nitrogen sources, resulting in improved cell density and plasmid yield.
This dehydrated agar formulation combines tryptone, yeast extract, sodium chloride, and agar to support robust bacterial metabolism and stable solid-phase culture performance. It is widely used in cloning workflows, phage display systems, recombinant DNA amplification, and protein expression studies.
Specification
| Total Product Size | 250 g |
| Container Size | 250 g |
| Number of Containers | 1 |
| Form | Dehydrated powder medium |
| Application | E. coli culture / Phage propagation / Cloning |
| Shipping Condition | Room Temperature (RT) |
| UNSPSC Code | 41106200 |
| Category | Microbiological Culture Media |
Features
- High-nutrient enriched formulation for enhanced E. coli growth and metabolic activity.
- Optimized for M13 filamentous phage replication and phage display systems.
- Supports high-efficiency plasmid amplification and recombinant DNA cloning workflows.
- Rich tryptone and yeast extract composition for improved bacterial density and protein yield.
- Stable agar-based solid medium for consistent plate preparation and reproducible results.
Applications
- Recombinant DNA cloning and plasmid amplification in E. coli.
- M13 filamentous phage propagation and phage display research.
- High-density bacterial culture for protein expression systems.
- Microbiological solid media preparation for laboratory research.
- Molecular biology workflows requiring rich bacterial growth medium.
- Genetic engineering and synthetic biology experiments.
Composition (per liter)
- Tryptone: 16.0 g
- Yeast Extract: 10.0 g
- Sodium Chloride: 5.0 g
- Agar: 15.0 g
Preparation Instructions
-
Dissolve 46 g of 2xYT Agar powder in 1 L distilled water.
-
Heat with stirring until fully dissolved and boiling.
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Autoclave at 121°C for 15 minutes for sterilization.
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Pour into sterile Petri dishes under aseptic conditions.
pH: 6.8 ± 0.2 at 25°C
Documents
COAFrequently Asked Questions
What is 2xYT Agar used for?
It is a rich microbiological culture medium used for high-density growth of E. coli and propagation of M13 bacteriophage in molecular biology and cloning workflows.
How is 2xYT different from LB medium?
2xYT contains higher concentrations of tryptone and yeast extract, providing more nutrients for improved bacterial growth, higher plasmid yield, and better phage production compared to LB.
Why is 2xYT preferred for phage display?
Its enriched nutrient composition supports efficient M13 phage replication and higher infection efficiency in E. coli host strains.
Can 2xYT Agar be used for recombinant protein expression?
Yes, it is widely used in recombinant protein expression systems due to its ability to support high cell density cultures.
How should 2xYT Agar be stored?
Store the dehydrated powder in a dry, tightly sealed container at room temperature away from moisture.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
